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GATAD2B is required for pre‐implantation embryonic development by regulating zygotic genome activation
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GATAD2B is required for pre‐implantation embryonic development by regulating zygotic genome activation
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GATAD2B is required for pre‐implantation embryonic development by regulating zygotic genome activation
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Journal Article
GATAD2B is required for pre‐implantation embryonic development by regulating zygotic genome activation
2024
Overview
Major zygotic genome activation (ZGA) occurs at the late 2‐cell stage and involves the activation of thousands of genes, supporting early embryonic development. The reasons underlying the regulation of ZGA are not clear. Acetylation modifications of histone tails promote transcriptional activation, and the maternal deletion of H4K16ac leads to failure in ZGA. GATAD2B is one of the core subunits of the nucleosome remodelling and histone deacetylation (NuRD) complex. Our research has shown that GATAD2B exhibits specific nucleus localization and high protein expression from the late 2‐cell stage to the 8‐cell stage. This intriguing phenomenon prompted us to investigate the relationship between GATAD2B and the ZGA. We discovered a distinctive pattern of GATAD2B, starting from the late 2‐cell stage with nuclear localization. GATAD2B depletion resulted in defective embryonic development, including increased DNA damage at morula, decreased blastocyst formation rate, and abnormal differentiation of ICM/TE lineages. Consistent with the delay during the cleavage stage, the transcriptome analysis of the 2‐cell embryo revealed inhibition of the cell cycle G2/M phase transition pathway. Furthermore, the GATAD2B proteomic data provided clear evidence of a certain association between GATAD2B and molecules involved in the cell cycle pathway. As hypothesized, GATAD2B‐deficient 2‐cell embryos exhibited abnormalities in ZGA during the maternal‐to‐embryonic transition, with lower expression of the major ZGA marker MERVL. Overall, our results demonstrate that GATAD2B is essential for early embryonic development, in part through facilitating ZGA. Major ZGA occurs at the late‐2 cell stage and involves the activation of thousands of genes, supporting early embryonic development. GATAD2B is one of the core subunits of the nucleosome remodelling and histone deacetylation (NuRD) complex. Our results demonstrate that GATAD2B is essential for pre‐implantation embryonic development, in part through facilitating ZGA.
