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Quantification of lipophilic toxins associated with diarrhetic shellfish poisoning in Japanese bivalves by liquid chromatography-mass spectrometry and comparison with mouse bioassay
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Quantification of lipophilic toxins associated with diarrhetic shellfish poisoning in Japanese bivalves by liquid chromatography-mass spectrometry and comparison with mouse bioassay
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Quantification of lipophilic toxins associated with diarrhetic shellfish poisoning in Japanese bivalves by liquid chromatography-mass spectrometry and comparison with mouse bioassay
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Journal Article
Quantification of lipophilic toxins associated with diarrhetic shellfish poisoning in Japanese bivalves by liquid chromatography-mass spectrometry and comparison with mouse bioassay
2005
Overview
: Quantification of lipophilic toxins in bivalves associated with diarrhetic shellfish poisoning was investigated by liquid chromatography–mass spectrometry (LC–MS). Using a C8‐silica reversed phase column and a mobile phase of aqueous acetonitrile containing 2 mM ammonium formate and 50 mM formic acid, okadaic acid, dinophysistoxin‐1, 7‐O‐palmitoyldinophysistoxin‐1, pectenotoxin‐1, pectenotoxin‐2, pectenotoxin‐6, pectenotoxin‐2 seco‐acid, yessotoxin, and 45‐hydroxyyessotoxin in bivalves were quantified by LC–MS in the negative mode. When the crude 90% methanol extracts were analyzed by LC–MS, there were no significant effects from bivalve matrices on the quantification of toxins. More than 200 bivalve samples collected from various production areas in Japan were analyzed by LC–MS. Pectenotoxin‐6 and dinophysistoxin‐1 were the dominant toxins in scallops and mussels, respectively. Yessotoxin and 45‐hydroxyyessotoxin were also detected in both species. Comparison of the quantitative results obtained for these bivalve samples between LC–MS and mouse bioassay indicates that LC–MS is suitable for routine monitoring of lipophilic toxins in Japanese bivalves.
Publisher
Blackwell Science Pty,Springer Nature B.V
Subject
/ BIVALVIA
/ BIVALVO
/ DIARREA
/ DIARRHEE
/ diarrhetic shellfish poisoning
/ ENFERMEDADES TRANSM POR ALIMENTOS
/ HPLC
/ Ions
/ liquid chromatography-mass spectrometry
/ MALADIE TRANSMISSIBLE PAR ALIMENT
/ Marine
/ Methanol
/ MICE
/ Mollusks
/ RATON
/ Silica
/ SOURIS
/ TOXINAS
/ TOXINE
/ TOXINS
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