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Arthropod transcriptional activator protein-1 (AP-1) aids tick-rickettsial pathogen survival in the cold
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Arthropod transcriptional activator protein-1 (AP-1) aids tick-rickettsial pathogen survival in the cold
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Journal Article
Arthropod transcriptional activator protein-1 (AP-1) aids tick-rickettsial pathogen survival in the cold
2018
Overview
Ixodes scapularis
ticks transmit several pathogens to humans including rickettsial bacterium,
Anaplasma phagocytophilum
. Here, we report that
A
.
phagocytophilum
uses tick transcriptional activator protein-1 (AP-1) as a molecular switch in the regulation of arthropod antifreeze gene,
iafgp
. RNAi-mediated silencing of
ap-1
expression significantly affected
iafgp
gene expression and
A
.
phagocytophilum
burden in ticks upon acquisition from the murine host. Gel shift assays provide evidence that both the bacterium and AP-1 influences
iafgp
promoter and expression. The luciferase assays revealed that a region of approximately 700 bp upstream of the antifreeze gene is sufficient for AP-1 binding to promote
iafgp
gene expression. Furthermore, survival assays revealed that AP-1-deficient ticks were more susceptible to cold in comparison to the mock controls. In addition, this study also indicates arthropod AP-1 as a global regulator for some of the tick genes critical for
A
.
phagocytophilum
survival in the vector. In summary, our study defines a novel mode of arthropod signaling for the survival of both rickettsial pathogen and its medically important vector in the cold.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/89
/ 13/95
/ 14
/ 38
/ 38/22
/ 38/44
/ 38/71
/ 38/77
/ 45
/ 45/29
/ 59
/ Anaplasma phagocytophilum - physiology
/ Animals
/ Female
/ Humanities and Social Sciences
/ Mice
/ Open Reading Frames - genetics
/ Promoter Regions, Genetic - genetics
/ Science
/ Survival
/ Transcription Factor AP-1 - chemistry
/ Transcription Factor AP-1 - genetics
