Journal Article

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer

Chen, Hong-Fang, Zhang, Hui, Gao, Peng, Zhang, Guo-Hao, Tian, Ya-Ru, Ma, Ran-Ran

2021

Overview

Gastric cancer (GC) is one of the most common malignant neoplasms. Invasion and metastasis are the main causes of GC-related deaths. Recently, kinesins were discovered to be involved in tumor development. The aim of this study was to elucidate the roles of kinesin superfamily protein 26A (KIF26A) in GC and its underlying molecular mechanism in regulating tumor invasion and metastasis. Using real-time quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC), we showed that KIF26A expression was lower in GC tissues without lymph node metastasis (LNM) than in nontumorous gastric mucosa, and even lower in GC tissues with LNM than in GC tissues without LNM. Functional experiments showed that KIF26A inhibited migration and invasion of GC cells. We further identified focal-adhesion kinase (FAK), phosphatidylinositol 3-kinase regulatory subunit alpha (PI3KR1), VAV3, Rac1 and p21-activated kinase 2, and β-PAK (PAK3) as downstream effectors of KIF26A in the focal-adhesion pathway, and we found that KIF26A could regulate FAK mRNA expression through inhibiting c-MYC by MAPK pathway. c-MYC could bind to the promoter of FAK and activate FAK transcription. Moreover, we found that KIF26A-mediated inactivation of the focal-adhesion pathway could reduce the occurrence of the epithelial-to-mesenchymal transition (EMT) by increasing expression of E-cadherin and reducing that of Snail. Luciferase assays and Western blotting revealed that miR-19a and miR-96 negatively regulate KIF26A. Finally, we found that decreased expression of KIF26A has been positively correlated with histological differentiation, Lauren classification, LNM, distal metastasis, and clinical stage, as well as poor survival in patients with GC. These data indicate that KIF26A could inhibit GC migration and invasion by regulating the focal-adhesion pathway and repressing the occurrence of EMT.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 1-Phosphatidylinositol 3-kinase / 14/1 / 38/109 / 38/89 / 42/89 / 45/47 / 631/67/1504/1829 / 631/67/322 / Animals / Apoptosis / c-Myc protein / Cancer / Cell Biology / Cell Line, Tumor / Cellular signal transduction / Development and progression / Disease Models, Animal / E-cadherin / Female / Focal adhesion kinase / Focal Adhesion Kinase 1 - metabolism / Gastric cancer / Gastric mucosa / Gene expression / Genetic aspects / Health aspects / Heterografts / Human Genetics / Humans / Immunohistochemistry / Internal Medicine / Kinases / Kinesin / Lymph nodes / Male / MAP kinase / Medicine / Medicine & Public Health / Mesenchyme / Metastases / Metastasis / Mice / Mice, Inbred NOD / Mice, SCID / MicroRNA / MicroRNAs - metabolism / Myc protein / Neoplasia / Neoplasm Metastasis / Oncology / Oncology, Experimental / p21-activated kinase / Polymerase chain reaction / Protein kinases / Rac1 protein / Signal Transduction / Stomach cancer / Stomach Neoplasms - metabolism / Stomach Neoplasms - pathology / Transcription / Western blotting