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Transcriptional responses and secondary metabolites variation of tomato plant in response to tobacco mosaic virus infestation
by
Younes, Hosny A.
, Abdelkhalek, Ahmed
, Aseel, Dalia G.
, Behiry, Said I.
, Rabie, Mona
in
631/326/596
/ 631/449/1659
/ 631/449/2169
/ 631/449/2661
/ 631/449/2667
/ Amino acid sequence
/ Chlorogenic acid
/ Coat protein
/ Ferulic acid
/ Flavonoids
/ Flavonoids - metabolism
/ GC–MS
/ Gene expression
/ Gene Expression Regulation, Plant
/ HPLC
/ Humanities and Social Sciences
/ Infections
/ Inoculation
/ Metabolites
/ multidisciplinary
/ Naringenin
/ Phenols
/ Plant Diseases - genetics
/ Plant Diseases - virology
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant virus diseases
/ Plant viruses
/ Polyphenolic compounds
/ Polyphenols
/ Pyrogallol
/ Rutin
/ Salicylic acid
/ Science
/ Science (multidisciplinary)
/ Secondary Metabolism
/ Secondary metabolites
/ Sequence analysis
/ Solanum lycopersicum
/ Solanum lycopersicum - genetics
/ Solanum lycopersicum - metabolism
/ Solanum lycopersicum - virology
/ Tobacco mosaic virus
/ Tobacco Mosaic Virus - physiology
/ Tobamovirus
/ Tomato
/ Tomatoes
/ Viral infections
2024
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Transcriptional responses and secondary metabolites variation of tomato plant in response to tobacco mosaic virus infestation
by
Younes, Hosny A.
, Abdelkhalek, Ahmed
, Aseel, Dalia G.
, Behiry, Said I.
, Rabie, Mona
in
631/326/596
/ 631/449/1659
/ 631/449/2169
/ 631/449/2661
/ 631/449/2667
/ Amino acid sequence
/ Chlorogenic acid
/ Coat protein
/ Ferulic acid
/ Flavonoids
/ Flavonoids - metabolism
/ GC–MS
/ Gene expression
/ Gene Expression Regulation, Plant
/ HPLC
/ Humanities and Social Sciences
/ Infections
/ Inoculation
/ Metabolites
/ multidisciplinary
/ Naringenin
/ Phenols
/ Plant Diseases - genetics
/ Plant Diseases - virology
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant virus diseases
/ Plant viruses
/ Polyphenolic compounds
/ Polyphenols
/ Pyrogallol
/ Rutin
/ Salicylic acid
/ Science
/ Science (multidisciplinary)
/ Secondary Metabolism
/ Secondary metabolites
/ Sequence analysis
/ Solanum lycopersicum
/ Solanum lycopersicum - genetics
/ Solanum lycopersicum - metabolism
/ Solanum lycopersicum - virology
/ Tobacco mosaic virus
/ Tobacco Mosaic Virus - physiology
/ Tobamovirus
/ Tomato
/ Tomatoes
/ Viral infections
2024
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Transcriptional responses and secondary metabolites variation of tomato plant in response to tobacco mosaic virus infestation
by
Younes, Hosny A.
, Abdelkhalek, Ahmed
, Aseel, Dalia G.
, Behiry, Said I.
, Rabie, Mona
in
631/326/596
/ 631/449/1659
/ 631/449/2169
/ 631/449/2661
/ 631/449/2667
/ Amino acid sequence
/ Chlorogenic acid
/ Coat protein
/ Ferulic acid
/ Flavonoids
/ Flavonoids - metabolism
/ GC–MS
/ Gene expression
/ Gene Expression Regulation, Plant
/ HPLC
/ Humanities and Social Sciences
/ Infections
/ Inoculation
/ Metabolites
/ multidisciplinary
/ Naringenin
/ Phenols
/ Plant Diseases - genetics
/ Plant Diseases - virology
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant virus diseases
/ Plant viruses
/ Polyphenolic compounds
/ Polyphenols
/ Pyrogallol
/ Rutin
/ Salicylic acid
/ Science
/ Science (multidisciplinary)
/ Secondary Metabolism
/ Secondary metabolites
/ Sequence analysis
/ Solanum lycopersicum
/ Solanum lycopersicum - genetics
/ Solanum lycopersicum - metabolism
/ Solanum lycopersicum - virology
/ Tobacco mosaic virus
/ Tobacco Mosaic Virus - physiology
/ Tobamovirus
/ Tomato
/ Tomatoes
/ Viral infections
2024
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Transcriptional responses and secondary metabolites variation of tomato plant in response to tobacco mosaic virus infestation
Journal Article
Transcriptional responses and secondary metabolites variation of tomato plant in response to tobacco mosaic virus infestation
2024
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Overview
The present study focused on the impact of infection with the tobacco mosaic virus (TMV). Specifically, changes in phytochemicals and gene activity related to pathogenesis-related and phenylpropanoid pathway genes in tomato plants (
Solanum lycopersicum
L.) during a period of 2–14 days post-inoculation (dpi). According to TEM investigation and coat protein sequence analysis, the purified TMV Egyptian AM isolate (PP133743) has a rod-shaped structure with a diameter of around 110 nm. The RT-qPCR analysis revealed that
PR-1
showed an initial increase after TMV infection, as seen in the time-course analysis. In contrast,
PR-2
was consistently elevated throughout the infection, suggesting a stronger reaction to the virus and suppressing
PAL
expression at 6 to 14 dpi. The expression levels of
HQT
and
CHS
transcripts exhibited alternating patterns of up-regulation and down-regulation at different time intervals. The HPLC and GC–MS analysis of control- and TMV-infected tomato extracts revealed that different phenolic, flavonoid, and fatty acid compounds were increased (such as naringenin, rutin, flavone, ferulic acid, and pyrogallol) or significantly decreased (such as salicylic acid and chlorogenic acid) after TMV infection. The ability of TMV to inhibit most polyphenolic compounds could potentially accelerate the viral life cycle. Consequently, focusing on enhancing the levels of such suppressed compounds may be critical for developing plant viral infection management strategies.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
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