Journal Article

Production and Characterization of a Novel Laccase with Cold Adaptation and High Thermal Stability from an Isolated Fungus

Li, Zhiling, Wang, Zhixin, Zhang, Dabing, Liao, Xiangru, Cai, Yujie, Zhang, Feng

2010

Overview

A new white-rot fungus SYBC-L1, which could produce an extracellular laccase, was isolated from a decayed Elaeocarpus sylvestris. The strain was identified as Pycnoporus sp. SYBC-L1 according to the morphological characteristics and ribosomal ITS1-5.8S-ITS2 RNA genomic sequence analysis. The highest laccase activity of 24.1 U ml⁻¹, which was approximately 40-fold than that in basal medium, was achieved in optimal culture medium in submerged fermentation. The laccase produced by Pycnoporus sp. SYBC-L1 was not only a cold adaptation enzyme with a relative catalytic activity of 30.2% at 0°C but also a high thermostable enzyme. The half-lives at 60, 70 and 80°C were 85.5, 37.2, and 2.6 h, respectively. The laccase could effectively decolorize weak acid blue AS and diamond black PV up to 88% and 74.7%, respectively, within 2 h in the absence of any redox mediators. The results suggested Pycnoporus sp. SYBC-L1 was a potential candidate for laccase production and industrial application.

• Publisher: New York : Humana Press Inc,Humana Press Inc,Springer,Springer Nature B.V
• Subject: Adaptation, Physiological / Animals / Biochemistry / Biological and medical sciences / biosynthesis / Biotechnology / Carbon / Carbon - pharmacology / Chemistry / Chemistry and Materials Science / Cold / Cold Temperature / Color / Coloring Agents / Coloring Agents - metabolism / Copper / Copper - pharmacology / culture media / decolorization / drug effects / dyes / Elaeocarpaceae / Elaeocarpaceae - microbiology / Elaeocarpus / Elaeocarpus sylvestris / Enzyme Stability / Enzymes / enzymology / Fermentation / Fermentation - drug effects / Fundamental and applied biological sciences. Psychology / Fungi / half life / Heat / Hydrogen-Ion Concentration / Immersion / industrial microbiology / isolation / isolation & purification / laccase / Laccase - biosynthesis / Laccase - chemistry / Laccase - metabolism / metabolism / microbiology / Nitrogen / Nitrogen - pharmacology / optimization / pharmacology / physiology / Pycnoporus / Pycnoporus - drug effects / Pycnoporus - enzymology / Pycnoporus - isolation & purification / Pycnoporus - physiology / ribosomal RNA / submerged fermentation / temperature / thermal stability / white-rot fungi / Wool