Journal Article

Exploring the dynamic relationship between antimicrobial resistance, virulence fitness, and host responses in Listeria monocytogenes infections

Algarzae, Norah K., Elazab, Sara T., Alamri, Abdulhakeem S., Fakhary, Basma A., Alharbi, Nada K., Bendary, Mahmoud M., Alsahly, Musaad B., El-Hamid, Marwa I. Abd, Mosbah, Rasha A., Alsufayan, Thamer A., Elmanakhly, Arwa R., El-Tarabili, Reham M., Alhomrani, Majid, Alharbi, Maha, Awad, Naglaa F. S.

2025

Overview

Listeriosis is a severe zoonotic disease caused by Listeria monocytogenes ( L. monocytogenes ), which can be acquired through animal source foods. This pathogen shows unique virulence fitness allowing it to penetrate and survive inside host cells causing extremely dangerous symptoms. Therefore, we aimed to correlate the clinical outcomes with the antimicrobial resistance and virulence profiles of L. monocytogenes . This is crucial for improving disease management, developing new therapeutic strategies, enhancing public health and food safety, and advancing scientific knowledge. Therefore, we assessed the antimicrobial resistance and virulence profiles of L. monocytogenes isolated from various sources, along with their potential to cause disease, using an in vivo rabbit model. Based on identification criteria, 47 L. monocytogenes isolates (15.7%) were recovered with the highest detection rates among rabbits (22%). Unfortunately, all the investigated isolates showed multidrug resistance (MDR) as well as multi-virulent profiles with 45 highly heterogeneous clusters. Noteworthy, the degree of illnesses of the experimentally infected rabbits was dependent on the virulence profiles. Specific nervous manifestations and severe histopathological alterations were observed in experimental rabbits infected with highly virulent isolates confirming that the potential ability of this pathogen to produce a disease is not always decipherable from its virulence arrays. Finally, it was confirmed that managing infections caused by L. monocytogenes has become increasingly challenging due to its high antimicrobial resistance, strong virulence, and the severe pathological effects linked to its virulence factors.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/326/107 / 631/326/325 / Animal-based foods / Animals / Anti-Bacterial Agents - pharmacology / Antibiotics / Antimicrobial agents / Antimicrobial resistance / Biofilms / Disease Models, Animal / Drug resistance / Drug Resistance, Bacterial / Drug Resistance, Multiple, Bacterial / Encephalitis / Ethics / Food safety / Food sources / Foodborne pathogens / Genes / Genetic engineering / Host-Pathogen Interactions / Humanities and Social Sciences / Humans / Infections / Investigations / L. monocytogenes / Listeria / Listeria monocytogenes / Listeria monocytogenes - drug effects / Listeria monocytogenes - isolation & purification / Listeria monocytogenes - pathogenicity / Listeriosis / Listeriosis - drug therapy / Listeriosis - microbiology / Listeriosis - pathology / Meningitis / Microbial Sensitivity Tests / Milk / Morphology / Motility / multidisciplinary / Multidrug resistance / Pathogenicity / Pathogens / Public health / Rabbits / Resistance / Science / Science (multidisciplinary) / Sepsis / Sheep / Surveillance / Virulence / Virulence factors / Zoonoses / Zoonotic