Journal Article

Interaction between Mas1 and AT1RA contributes to enhancement of skeletal muscle angiogenesis by angiotensin-(1-7) in Dahl salt-sensitive rats

Exner, Eric C., Zimmermann, Michael, Stodola, Timothy, Dsouza, Nikita R., Greene, Andrew S., Hoffmann, Brian R., Geurts, Aron M., Casati, Marc, Lombard, Julian H.

2020

Overview

The heptapeptide angiotensin-(1-7) (Ang-(1-7)) is protective in the cardiovascular system through its induction of vasodilator production and angiogenesis. Despite acting antagonistically to the effects of elevated, pathophysiological levels of angiotensin II (AngII), recent evidence has identified convergent and beneficial effects of low levels of both Ang-(1-7) and AngII. Previous work identified the AngII receptor type I (AT1R) as a component of the protein complex formed when Ang-(1-7) binds its receptor, Mas1. Importantly, pharmacological blockade of AT1R did not alter the effects of Ang-(1-7). Here, we use a novel mutation of AT1RA in the Dahl salt-sensitive (SS) rat to test the hypothesis that interaction between Mas1 and AT1R contributes to proangiogenic Ang-(1-7) signaling. In a model of hind limb angiogenesis induced by electrical stimulation, we find that the restoration of skeletal muscle angiogenesis in SS rats by Ang-(1-7) infusion is impaired in AT1RA knockout rats. Enhancement of endothelial cell (EC) tube formation capacity by Ang-(1-7) is similarly blunted in AT1RA mutant ECs. Transcriptional changes elicited by Ang-(1-7) in SS rat ECs are altered in AT1RA mutant ECs, and tandem mass spectrometry-based proteomics demonstrate that the protein complex formed upon binding of Ang-(1-7) to Mas1 is altered in AT1RA mutant ECs. Together, these data support the hypothesis that interaction between AT1R and Mas1 contributes to proangiogenic Ang-(1-7) signaling.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Angiogenesis / Angiotensin / Angiotensin I - metabolism / Angiotensin II / Animals / Biology and Life Sciences / Cardiovascular system / Drug dosages / Electric Stimulation / Electrical stimuli / Endothelial cells / Gene expression / Homeostasis / Hypertension / Hypotheses / Kinases / Laboratories / Levels / Male / Mass Spectrometry / Mass spectroscopy / Medical schools / Medicine and Health Sciences / Models, Animal / Muscle, Skeletal - blood supply / Muscle, Skeletal - metabolism / Muscles / Musculoskeletal system / Mutants / Mutation / Neovascularization, Physiologic / Peptide Fragments - metabolism / Peptides / Physiology / Precision medicine / Proteins / Proteomics / Proto-Oncogene Mas / Proto-Oncogene Proteins - metabolism / Rats / Rats, Inbred Dahl / Receptor, Angiotensin, Type 1 - genetics / Receptor, Angiotensin, Type 1 - metabolism / Receptors / Receptors, G-Protein-Coupled - metabolism / Research and Analysis Methods / Restoration / Salt / Scientific imaging / Signal Transduction / Signaling / Skeletal muscle / Transcription