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Evolutionary-driven C-MYC gene expression in mammalian fibroblasts
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Evolutionary-driven C-MYC gene expression in mammalian fibroblasts
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Evolutionary-driven C-MYC gene expression in mammalian fibroblasts
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Journal Article
Evolutionary-driven C-MYC gene expression in mammalian fibroblasts
2020
Overview
The extent to which mammalian cells share similar transcriptomes remains unclear. Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the dissection of gene regulatory landscapes. Therefore, the work was aimed to determine
C-MYC
relative expression across mammalian fibroblasts (
Ovis aries
and
Bos taurus
) via cross-species RT-qPCR and comprehensively explore its regulatory landscape by in silico tools. The prediction of transcription factor binding sites in
C-MYC
and its 2.5 kb upstream sequence revealed substantial variation, thus indicating evolutionary-driven re-wiring of cis-regulatory elements.
C-MYC
and its downstream target
TBX3
were up-regulated in
Bos taurus
fibroblasts. The relative expression of
C-MYC
regulators [
RONIN
(also known as
THAP11
),
RXRβ
, and
TCF3
] and the
C-MYC
-associated transcript elongation factor
CDK9
did not differ between species. Additional in silico analyses suggested
Bos taurus
-specific
C-MYC
exonization, alternative splicing, and binding sites for non-coding RNAs.
C-MYC
protein orthologs were highly conserved, while variation was in the transactivation domain and the leucine zipper motif. Altogether, mammalian fibroblasts display evolutionary-driven
C-MYC
relative expression that should be instructive for understanding cellular physiology, cellular reprogramming, and
C-MYC
-related diseases.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 631/181
/ 631/208
/ 631/337
/ 631/532
/ 631/61
/ 631/67
/ 631/80
/ Animals
/ Cyclin-Dependent Kinase 9 - genetics
/ Humanities and Social Sciences
/ Mammals
/ Protein Processing, Post-Translational
/ Proto-Oncogene Proteins c-myc - genetics
/ Proto-Oncogene Proteins c-myc - metabolism
/ Regulatory Elements, Transcriptional
/ Science
/ Sequence Homology, Amino Acid
/ Sheep, Domestic - metabolism
/ Species
