Journal Article

Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes Simplex Virus Type 2 Infection

Baska, Piotr, Krzyzowska, Malgorzata, Donten, Mikolaj, Gniadek, Marianna, Sokolowska, Justyna, Celichowski, Grzegorz, Tomaszewska, Emilia, Nowak, Zuzanna, Nowakowska, Julita, Orlowski, Piotr, Grobelny, Jaroslaw

2014

Overview

The interaction between silver nanoparticles and herpesviruses is attracting great interest due to their antiviral activity and possibility to use as microbicides for oral and anogenital herpes. In this work, we demonstrate that tannic acid modified silver nanoparticles sized 13 nm, 33 nm and 46 nm are capable of reducing HSV-2 infectivity both in vitro and in vivo. The antiviral activity of tannic acid modified silver nanoparticles was size-related, required direct interaction and blocked virus attachment, penetration and further spread. All tested tannic acid modified silver nanoparticles reduced both infection and inflammatory reaction in the mouse model of HSV-2 infection when used at infection or for a post-infection treatment. Smaller-sized nanoparticles induced production of cytokines and chemokines important for anti-viral response. The corresponding control buffers with tannic acid showed inferior antiviral effects in vitro and were ineffective in blocking in vivo infection. Our results show that tannic acid modified silver nanoparticles are good candidates for microbicides used in treatment of herpesvirus infections.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Animals / Anogenital / Antiviral activity / Antiviral agents / Antiviral Agents - chemistry / Antiviral Agents - pharmacology / Antiviral drugs / Biology and Life Sciences / Cell Line / Chemistry / Chemokines / Chlorocebus aethiops / Cytokines / Cytokines - metabolism / Disease Models, Animal / Dose-Response Relationship, Drug / Engineering and Technology / Epidemiology / Female / Herpes simplex / Herpes Simplex - drug therapy / Herpes Simplex - metabolism / Herpes Simplex - virology / Herpes viruses / Herpesvirus 2, Human - drug effects / Infections / Infectivity / Inflammation / Inflammation - drug therapy / Inflammation - metabolism / Inflammation - virology / Life sciences / Medicine and Health Sciences / Metal Nanoparticles - administration & dosage / Metal Nanoparticles - chemistry / Metal Nanoparticles - ultrastructure / Mice / Microbial Sensitivity Tests / Microbicides / Nanoparticles / Nervous system / Nitrates / Silver / Silver - chemistry / Silver - pharmacology / Sodium / Tannic acid / Tannins - chemistry / Tumor necrosis factor-TNF / Veterinary medicine / Viral infections / Virus attachment / Virus Attachment - drug effects / Virus Internalization - drug effects / Viruses