Journal Article

Association of germline variants with KRAS-mutation status in colorectal cancer

Pearlman, Rachel, Hullar, Meredith A. J., Georgeson, Peter, Schmit, Stephanie L., Um, Caroline Y., French, Amy J., Aglago, Elom K., Zaidi, Syed H., Gandhi, Tanish, Moreno, Victor, Bloomer, Amanda, Drew, David A., Schoen, Robert E., Huang, Wen-Yi, Trinh, Quang M., Phipps, Amanda I., Permuth, Jennifer B., Cao, Yin, Siegel, Erin M., Giannakis, Marios, Toland, Amanda Ewart, Figueiredo, Jane, Ramroop, Johnny, McElroy, Joseph Paul, Van Guelpen, Bethany, Nowak, Jonathan A., Tsilidis, Konstantinos, Obón-Santacana, Mireia, Berndt, Sonja I., Giles, Graham G., Hampel, Heather, Buchanan, Daniel D., Steinfelder, Robert S., Dauch, Cara, Gomez, Maria, Newton, Christina C., Ogino, Shuji, Stevens, Patrick, Gruber, Stephen B., Peters, Ulrike, Tjader, Nijole Pollock, Teer, Jamie K., Ugai, Tomotaka, Dimou, Niki, Hoffmeister, Michael, Peoples, Anita R., Campbell, Peter T., Cheng, Iona, Thomas, Claire E., Meadows, Owen, Brenner, Hermann, Loroña, Nicole, Huyghe, Jeroen R., Sun, Wei, Pellatt, Andrew, Chan, Andrew T.

2026

Overview

Somatic mutations in KRAS are a common driver of colorectal cancer (CRC) and present at different frequencies by race, sex, tumor site, ethnicity, and genetic similarity. Inherited germline variants may influence tumor somatic mutation frequency by altering mutation or DNA repair processes or altering cellular, immunological and/or microenvironmental responses after a mutation. We hypothesized that the germline genetic background modifies somatic KRAS mutation frequency in CRC. To test this, we performed a genome-wide association study (GWAS) in 7071 individuals with CRC, using KRAS mutation status as the phenotype. Single-nucleotide variants were chosen for validation analyses based on P values from the discovery GWAS, predicted in silico functional effects, and proximity to genes with potential cancer relevance. A validation analysis of 101 SNVs of interest was performed in 2482 individuals. No SNVs were significantly associated with KRAS -mutant CRC ( P value < 0.0005). One variant rs73067863-T showed a non-significant exploratory association with fewer KRAS -mutant tumors in the combined sample ( P value = 9.7 × 10 –7 , OR = 0.75). Follow-up studies are needed to determine if these or other germline variants impact population differences in KRAS mutations in CRC.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/208/205 / 631/208/69 / 631/67/1504/1885 / 631/67/2324 / 631/67/395 / 631/67/69 / Adult / Aged / Antigens / Cancer / Colorectal cancer / Colorectal Neoplasms - genetics / Colorectal Neoplasms - pathology / Datasets / DNA repair / Ethnicity / Female / Genetic Predisposition to Disease / Genome-wide association studies / Genome-Wide Association Study / Genomes / Germ-Line Mutation / Germline variants / GWAS / Hispanic Americans / Humanities and Social Sciences / Humans / K-Ras protein / KRAS / Male / Middle Aged / multidisciplinary / Mutants / Mutation / Phenotypes / Polymorphism, Single Nucleotide / Population / Principal components analysis / Proto-Oncogene Proteins p21(ras) - genetics / Science / Science (multidisciplinary) / Self report / Somatic mutations / Tumors