Asset Details
Do you wish to reserve the book?
The natural function of the malaria parasite’s chloroquine resistance transporter
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
The natural function of the malaria parasite’s chloroquine resistance transporter
Do you wish to request the book?
The natural function of the malaria parasite’s chloroquine resistance transporter
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
The natural function of the malaria parasite’s chloroquine resistance transporter
2020
Overview
The
Plasmodium falciparum
chloroquine resistance transporter (PfCRT) is a key contributor to multidrug resistance and is also essential for the survival of the malaria parasite, yet its natural function remains unresolved. We identify host-derived peptides of 4-11 residues, varying in both charge and composition, as the substrates of PfCRT in vitro and in situ, and show that PfCRT does not mediate the non-specific transport of other metabolites and/or ions. We find that drug-resistance-conferring mutations reduce both the peptide transport capacity and substrate range of PfCRT, explaining the impaired fitness of drug-resistant parasites. Our results indicate that PfCRT transports peptides from the lumen of the parasite’s digestive vacuole to the cytosol, thereby providing a source of amino acids for parasite metabolism and preventing osmotic stress of this organelle. The resolution of PfCRT’s native substrates will aid the development of drugs that target PfCRT and/or restore the efficacy of existing antimalarials.
Plasmodium falciparum
chloroquine resistance transporter (PfCRT) mediates multidrug resistance, but its natural function remains unclear. Here, Shafik et al. show that PfCRT transports host-derived peptides of 4-11 residues but not other ions or metabolites, and that drug-resistance-conferring PfCRT mutants have reduced peptide transport.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14
/ 14/1
/ 14/63
/ 64
/ 64/114
/ 82/1
/ 82/58
/ 82/80
/ 9/10
/ Animals
/ Antimalarials - pharmacology
/ Biological Transport, Active
/ Cytosol
/ Female
/ Host-Parasite Interactions - genetics
/ Host-Parasite Interactions - physiology
/ Humanities and Social Sciences
/ Humans
/ Ions
/ Malaria
/ Malaria, Falciparum - drug therapy
/ Malaria, Falciparum - metabolism
/ Malaria, Falciparum - parasitology
/ Membrane Transport Proteins - genetics
/ Membrane Transport Proteins - metabolism
/ Mutant Proteins - metabolism
/ Mutation
/ Peptides
/ Plasmodium falciparum - drug effects
/ Plasmodium falciparum - genetics
/ Plasmodium falciparum - metabolism
/ Protozoan Proteins - genetics
/ Protozoan Proteins - metabolism
/ Residues
/ Science
