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Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair
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Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair
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Journal Article
Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair
2021
Overview
How adhesive forces are transduced and integrated into biochemical signals at focal adhesions (FAs) is poorly understood. Using cells adhering to deformable micropillar arrays, we demonstrate that traction force and FAK localization as well as traction force and Y397-FAK phosphorylation are linearly coupled at individual FAs on stiff, but not soft, substrates. Similarly, FAK phosphorylation increases linearly with external forces applied to FAs using magnetic beads. This mechanosignaling coupling requires actomyosin contractility, talin-FAK binding, and full-length vinculin that binds talin and actin. Using an in vitro 3D biomimetic wound healing model, we show that force-FAK signaling coupling coordinates cell migration and tissue-scale forces to promote microtissue repair. A simple kinetic binding model of talin-FAK interactions under force can recapitulate the experimental observations. This study provides insights on how talin and vinculin convert forces into FAK signaling events regulating cell migration and tissue repair.
How adhesive forces are transduced and integrated into biochemical signals at focal adhesions (FAs) is poorly understood. Here authors show that force- FAK signaling coupling coordinates cell migration and tissue-scale forces to promote microtissue repair.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/106
/ 14/19
/ Actin
/ Animals
/ Beads
/ Binding
/ Coupling
/ Focal Adhesion Kinase 1 - deficiency
/ Focal Adhesion Kinase 1 - genetics
/ Focal Adhesion Kinase 1 - metabolism
/ Focal Adhesions - metabolism
/ Humanities and Social Sciences
/ Mechanotransduction, Cellular
/ Mice
/ Repair
/ RNA, Small Interfering - genetics
/ Science
/ Talin
/ Talin - antagonists & inhibitors
/ Traction
/ Vinculin
