Journal Article

Conformational dynamics of the Beta and Kappa SARS-CoV-2 spike proteins and their complexes with ACE2 receptor revealed by cryo-EM

Liu, Caixuan, Xu, Shiqi, Zhang, Chao, Zuo, Qinyu, Cong, Yao, Li, Zuyang, Huang, Zhong, Hong, Qin, Wang, Yifan, Xu, Cong, Wang, Yanxing

2021

Overview

The emergence of SARS-CoV-2 Kappa and Beta variants with enhanced transmissibility and resistance to neutralizing antibodies has created new challenges for the control of the ongoing COVID-19 pandemic. Understanding the structural nature of Kappa and Beta spike (S) proteins and their association with ACE2 is of significant importance. Here we present two cryo-EM structures for each of the Kappa and Beta spikes in the open and open-prone transition states. Compared with wild-type (WT) or G614 spikes, the two variant spikes appear more untwisted/open especially for Beta, and display a considerable population shift towards the open state as well as more pronounced conformational dynamics. Moreover, we capture four conformational states of the S-trimer/ACE2 complex for each of the two variants, revealing an enlarged conformational landscape for the Kappa and Beta S-ACE2 complexes and pronounced population shift towards the three RBDs up conformation. These results implicate that the mutations in Kappa and Beta may modify the kinetics of receptor binding and viral fusion to improve virus fitness. Combined with biochemical analysis, our structural study shows that the two variants are enabled to efficiently interact with ACE2 receptor despite their sensitive ACE2 binding surface is modified to escape recognition by some potent neutralizing MAbs. Our findings shed new light on the pathogenicity and immune evasion mechanism of the Beta and Kappa variants. Here, the authors provide insights into the conformational dynamics of the Beta and Kappa SARS-CoV-2 spike (S) proteins by determining their cryo-EM structures, which revealed a distribution shift towards the open state for both variants compared to the wild-type S protein. They also present the structures of the Kappa and Beta S-ACE2 complexes, where a population shift towards the three receptor-binding domain up conformation was observed. In combination with biochemical data these structures show how the S protein variants efficiently recognize and bind to ACE2.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 101/28 / 42 / 631/326/596/4130 / 631/45/535/1258/1259 / 82/1 / 82/80 / 82/83 / ACE2 / Angiotensin-converting enzyme 2 / Angiotensin-Converting Enzyme 2 - chemistry / Antibodies / Antibodies, Neutralizing - immunology / Binding / Binding Sites / Biochemical analysis / COVID-19 / Cryoelectron Microscopy / Humanities and Social Sciences / Humans / Kinetics / Molecular Conformation / multidisciplinary / Mutation / Neutralizing / Pandemics / Pathogenicity / Pathogens / Protein Binding / Protein structure / Proteins / Receptors / SARS-CoV-2 / Science / Science (multidisciplinary) / Severe acute respiratory syndrome coronavirus 2 / Spike Glycoprotein, Coronavirus - chemistry / Trimers / Viral diseases