Journal Article

The genome-wide impact of trisomy 21 on DNA methylation and its implications for hematopoiesis

Vyas, Paresh, Myint, Swe Swe, Li, Shaobo, Ma, Xiaomei, Pandey, Priyatama, Muskens, Ivo S., Schraw, Jeremy M., Lupo, Philip J., Walsh, Kyle M., Siegmund, Kimberly D., de Bruijn, Marella F. T. R., Elliot, Natalina, Anguiano, Joaquin, Hansen, Helen M., Roberts, Irene, Jackson, Thomas, Roy, Ritu, Goudevenou, Katerina, de Smith, Adam J., Wiemels, Joseph L., Roy, Anindita

2021

Overview

Down syndrome is associated with genome-wide perturbation of gene expression, which may be mediated by epigenetic changes. We perform an epigenome-wide association study on neonatal bloodspots comparing 196 newborns with Down syndrome and 439 newborns without Down syndrome, adjusting for cell-type heterogeneity, which identifies 652 epigenome-wide significant CpGs ( P  < 7.67 × 10 −8 ) and 1,052 differentially methylated regions. Differential methylation at promoter/enhancer regions correlates with gene expression changes in Down syndrome versus non-Down syndrome fetal liver hematopoietic stem/progenitor cells ( P  < 0.0001). The top two differentially methylated regions overlap RUNX1 and FLI1 , both important regulators of megakaryopoiesis and hematopoietic development, with significant hypermethylation at promoter regions of these two genes. Excluding Down syndrome newborns harboring preleukemic GATA1 mutations ( N  = 30), identified by targeted sequencing, has minimal impact on the epigenome-wide association study results. Down syndrome has profound, genome-wide effects on DNA methylation in hematopoietic cells in early life, which may contribute to the high frequency of hematological problems, including leukemia, in children with Down syndrome. Down syndrome has a high co-morbidity with immune and hematopoietic disorders. Here, the authors perform an epigenome-wide association study in newborns with and without Down syndrome to find differential methylation across the genome, including in hematopoietic regulators RUNX1 and FLI1 .

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/31 / 38/77 / 38/91 / 45/23 / 45/61 / 631/136/232 / 631/1647/2210/2213 / 631/208/176/1988 / 692/308/174 / Case-Control Studies / Cells (biology) / Core Binding Factor Alpha 2 Subunit - genetics / Core Binding Factor Alpha 2 Subunit - metabolism / CpG Islands / Deoxyribonucleic acid / DNA / DNA Methylation / Down syndrome / Down Syndrome - genetics / Down Syndrome - metabolism / Down Syndrome - pathology / Down's syndrome / Epigenesis, Genetic / Epigenetics / Female / Fetus / Fetuses / GATA-1 protein / GATA1 Transcription Factor - genetics / GATA1 Transcription Factor - metabolism / Gene expression / Genome, Human / Genome-Wide Association Study / Genomes / Hematopoiesis / Hematopoiesis - genetics / Hematopoietic stem cells / Hematopoietic Stem Cells - metabolism / Hematopoietic Stem Cells - pathology / Hepatocytes / Heterogeneity / Humanities and Social Sciences / Humans / Infant, Newborn / Leukemia / Liver - metabolism / Liver - pathology / Male / Morbidity / multidisciplinary / Mutation / Neonates / Perturbation / Progenitor cells / Promoter Regions, Genetic / Proto-Oncogene Protein c-fli-1 - genetics / Proto-Oncogene Protein c-fli-1 - metabolism / Runx1 protein / Science / Science (multidisciplinary) / Trisomy