Journal Article

Discovery of an embryonically derived bipotent population of endothelial-macrophage progenitor cells in postnatal aorta

Nicholls, Stephen J., Pinto, Alexander R., Psaltis, Peter J., Toledo-Flores, Deborah, Bonder, Claudine S., Drummond, Grant R., Tan, Joanne T. M., Salagaras, Thalia, Kazenwadel, Jan, Williamson, Anna E., Hassanshahi, Mohammadhossein, Misra, Ashish, Vinh, Antony, Dimasi, Catherine, Martelotto, Luciano, Polo, Jose M., Harvey, Natasha L., Sharma, Shiwani, Liyanage, Sanuri, Schwarz, Nisha, Neufeld, Zoltan, Dona, Malathi S. I., Long, Aaron, Tran, Dang X. A., Fernando, Sanuja, Bursill, Christina A., Chandrakanthan, Vashe

2024

Overview

Converging evidence indicates that extra-embryonic yolk sac is the source of both macrophages and endothelial cells in adult mouse tissues. Prevailing views are that these embryonically derived cells are maintained after birth by proliferative self-renewal in their differentiated states. Here we identify clonogenic endothelial-macrophage (EndoMac) progenitor cells in the adventitia of embryonic and postnatal mouse aorta, that are independent of Flt3-mediated bone marrow hematopoiesis and derive from an early embryonic CX 3 CR1 + and CSF1R + source. These bipotent progenitors are proliferative and vasculogenic, contributing to adventitial neovascularization and formation of perfused blood vessels after transfer into ischemic tissue. We establish a regulatory role for angiotensin II, which enhances their clonogenic and differentiation properties and rapidly stimulates their proliferative expansion in vivo. Our findings demonstrate that embryonically derived EndoMac progenitors participate in local vasculogenic responses in the aortic wall by contributing to the expansion of endothelial cells and macrophages postnatally. The extraembryonic yolk sac is a major location for developmental hematopoiesis, but it is unclear whether non-bone marrow sources contribute during adulthood. Here they show that embryonically derived endothelial-macrophage progenitor cells located in the aorta are a bipotent source of macrophage and endothelial cells later in life.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/100 / 13/106 / 13/31 / 13/51 / 14/19 / 14/63 / 38/91 / 631/136/16 / 631/136/232/2059 / 631/532/1542 / 64/60 / Angiotensin / Angiotensin II / Animals / Aorta / Aorta - cytology / Blood vessels / Bone marrow / Cell Differentiation / Cell Proliferation / Cell self-renewal / Convergence / Coronary vessels / CX3C Chemokine Receptor 1 - genetics / CX3C Chemokine Receptor 1 - metabolism / CX3CR1 protein / Endothelial cells / Endothelial Cells - cytology / Endothelial Cells - metabolism / Female / fms-Like Tyrosine Kinase 3 / Hematopoiesis / Hematopoiesis - physiology / Hemopoiesis / Humanities and Social Sciences / Ischemia / Macrophages / Macrophages - cytology / Macrophages - metabolism / Male / Mice / Mice, Inbred C57BL / multidisciplinary / Neovascularization, Physiologic / Osteoprogenitor cells / Progenitor cells / Receptors, Chemokine - genetics / Receptors, Chemokine - metabolism / Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics / Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - metabolism / Science / Science (multidisciplinary) / Stem Cells - cytology / Stem Cells - metabolism / Vascularization / Yolk sac