Journal Article

Driver gene combinations dictate cutaneous squamous cell carcinoma disease continuum progression

Schoenherr, Christina, Bailey, Ulla-Maja, Stratigos, Alexander J., Treanor-Taylor, Mairi, Bone, Max, Harwood, Catherine A., Schreyer, Daniel, Thomson, Jason, Dimonitsas, Emmanouil, Wang, Jun, Bailey, Peter, Leigh, Irene M., Ridgway, Rachel A., Campbell, Andrew D., Sansom, Owen J., Purdie, Karin, Jackstadt, Rene, Cammareri, Patrizia, Blyth, Karen, Arron, Sarah T., Rickaby, William, Proby, Charlotte M., Inman, Gareth J.

2023

Overview

The molecular basis of disease progression from UV-induced precancerous actinic keratosis (AK) to malignant invasive cutaneous squamous cell carcinoma (cSCC) and potentially lethal metastatic disease remains unclear. DNA sequencing studies have revealed a massive mutational burden but have yet to illuminate mechanisms of disease progression. Here we perform RNAseq transcriptomic profiling of 110 patient samples representing normal sun-exposed skin, AK, primary and metastatic cSCC and reveal a disease continuum from a differentiated to a progenitor-like state. This is accompanied by the orchestrated suppression of master regulators of epidermal differentiation, dynamic modulation of the epidermal differentiation complex, remodelling of the immune landscape and an increase in the preponderance of tumour specific keratinocytes. Comparative systems analysis of human cSCC coupled with the generation of genetically engineered murine models reveal that combinatorial sequential inactivation of the tumour suppressor genes Tgfbr2 , Trp53 , and Notch1 coupled with activation of Ras signalling progressively drives cSCC progression along a differentiated to progenitor axis. Taken together we provide a comprehensive map of the cSCC disease continuum and reveal potentially actionable events that promote and accompany disease progression. The process by which actinic keratosis differentiates to malignant invasive cutaneous squamous cell carcinoma is unclear. Here, the authors use RNA-seq to illustrate a disease continuum between the two states, and use in vivo models to confirm the role of Tgfbr2, Trp53, and Notch1 in this process.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 38 / 38/91 / 631/67/1813/1352 / 631/67/70 / 64/60 / 82/51 / Animal models / Animals / Cancer / Carcinoma, Squamous Cell - genetics / Cell Differentiation / Combinatorial analysis / Differentiation / Disease Progression / DNA sequencing / Gene Expression Profiling / Genetic engineering / Humanities and Social Sciences / Humans / Inactivation / Invasiveness / Keratinocytes / Keratosis / Keratosis, Actinic / Metastases / Metastasis / Mice / multidisciplinary / Notch1 protein / Science / Science (multidisciplinary) / Skin Neoplasms - genetics / Squamous cell carcinoma / Systems analysis / Transcriptomics / Tumor suppressor genes / Tumors