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Mechanism of miR-200b-3p-induced FOSL2 inhibition of endometrial cancer cell proliferation and metastasis
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Mechanism of miR-200b-3p-induced FOSL2 inhibition of endometrial cancer cell proliferation and metastasis
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Journal Article
Mechanism of miR-200b-3p-induced FOSL2 inhibition of endometrial cancer cell proliferation and metastasis
2025
Overview
The purpose of this study was to investigate how miR-200b-3p inhibits the proliferation and metastasis of endometrial cancer cells by inducing the expression of FOSL2 in the AP1 transcription family. Endometrial cancer cell line HEC-1-A was divided into 16 groups: NC-mimic (transfected with negative control NC mimic), miR-200b-3p mimic (transfected with miR-200b-3p mimic), NC-suppress (transfected with negative control NC inhibit), miR-200b-3p inhibit group (transfected with miR-200b-3p inhibit), si-NC (transfected with negative control si-NC), si-FOSL2 (transfected with Si-FOSL2), oe-NC (transfected with negative control oe-NC), oe-FOSL2 group (oe-FOSL2), miR-200b-3p mimic + oe-NC group (co-transfected with miR-200b-3p mimic and oe-NC), miR-200b-3p mimic + oe-FOSL2 group (co-transfected with miR-200b-3p mimic and oe-FOSL2), miR-200b-3p inhibit + si-NC group (co-transfected with miR-200b-3p inhibit and si-NC), miR-200b-3p inhibit + si-FOSL2 group (co-transfected with miR-200b-3p inhibit and si-FOSL2), miR-200b-3p mimic + si-NC group (co-transfected with miR-200b-3p mimic and si-NC), miR-200b-3p mimic + si-FOSL2 group (co-transfected with miR-200b-3p mimic and si-FOSL2), miR-200b-3p inhibit + oe-NC group (co-transfected with miR-200b-3p inhibit and oe-NC), miR-200b-3p inhibit + oe-FOSL2 group (co-transfected with miR-200b-3p inhibit and oe-FOSL2). Real-time fluorescence quantitative PCR, Western blot, CCK-8 assay, scratch test and Transwell assay were used to detect the expression of miR-200b-3p mRNA, FOSL2 mRNA and protein, cell proliferation, migration and invasion. In endometrial cancer cell lines, the expression of miR-200b-3p was significantly down-regulated (
P
< 0.05), while the expression of FOSL2 was significantly up-regulated (
P
< 0.05). Compared with NC-mimic group, the expression of FOSL2, N-cadherin and Vimentin in miR-200b-3p mimic group was significantly decreased (
P
< 0.05), and the expression of E-cadherin was significantly increased (
P
< 0.05). The cell proliferation, migration rate and the number of transmembrane cells were significantly decreased (
P
< 0.05). Compared with the miR-200b-3p mimic + oe-NC group, the expression of FOSL2, N-cadherin and Vimentin in miR-200b-3p mimic + oe-FOSL2 was significantly increased (
P
< 0.05), the expression level of E-cadherin was significantly decreased (
P
< 0.05), and the cell proliferation, migration rate and the number of transmembrane cells were significantly increased (
P
< 0.05). Compared with NC-inhibit group, the expression of FOSL2, N-cadherin and Vimentin in miR-200b-3p inhibit group was significantly increased (
P
< 0.05), and the expression of E-cadherin was significantly decreased (
P
< 0.05). The cell proliferation, migration rate and the number of transmembrane cells were significantly increased (
P
< 0.05). Compared with the miR-200b-3p inhibit + si-NC group, the expression of FOSL2, N-cadherin and Vimentin in miR-200b-3p inhibit + si-FOSL2 was significantly decreased (
P
< 0.05), and the expression of E-cadherin was significantly increased (
P
< 0.05); the cell proliferation, migration rate and the number of transmembrane cells were significantly decreased (
P
< 0.05) The expression of miR-200b-3p in endometrial cancer cells is down-regulated, which can inhibit the proliferation, migration and invasion of endometrial cancer cells by regulating the EMT process, and its mechanism is related to its targeted negative regulation of FOSL2 expression.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/308
/ 692/53
/ Cancer
/ Cell Proliferation - genetics
/ EMT
/ Endometrial Neoplasms - genetics
/ Endometrial Neoplasms - metabolism
/ Endometrial Neoplasms - pathology
/ Female
/ Fos-Related Antigen-2 - genetics
/ Fos-Related Antigen-2 - metabolism
/ FOSL2
/ Gene Expression Regulation, Neoplastic
/ Humanities and Social Sciences
/ Humans
/ Science
/ Vimentin
