Journal Article

The bovine papillomavirus E6 oncoprotein interacts with paxillin and disrupts the actin cytoskeleton

Tong, Xiao, Howley, Peter M.

1997

Overview

The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) has been shown to transform cells through a p53-independent pathway, but its transforming mechanism is unknown. Here we demonstrate in vitro and in vivo interactions between BPV-1 E6 and the focal adhesion protein paxillin. The ability of BPV-1 E6 to complex with paxillin correlated with its ability to transform; E6 mutant proteins impaired in their transformation function also were impaired in their abilities to bind paxillin. E6 binding to paxillin also may contribute to the carcinogenic potential of the human papillomavirus (HPV); we were able to show in vitro binding of paxillin to the E6 proteins of the cancer-associated type HPV 16 but not of the nononcogenic types 6 and 11. The association of E6 with paxillin was affected by depolymerization of the actin fiber network, and overexpression of BPV-1 E6 led to disruption of actin fiber formation. Disruption of the actin cytoskeleton is a characteristic of many transformed cells, and, in BPV-1 transformed cells, may be mediated by BPV-1 E6 through its interaction with paxillin.

• Publisher: National Academy of Sciences of the United States of America,The National Academy of Sciences of the USA,National Academy of Sciences
• Subject: actin / ACTINA / ACTINE / Actins / Actins - physiology / Animals / Antibodies / ANTIMETABOLITE / ANTIMETABOLITOS / binding proteins / Biological Sciences / Bovine papillomavirus 1 / Bovine papillomavirus 1 - genetics / Cell Adhesion Molecules / Cell Adhesion Molecules - metabolism / Cell Biology / Cells / Cellular biology / COS Cells / cytochalasin D / Cytochalasin D - pharmacology / Cytochalasins / Cytoskeletal Proteins / Cytoskeletal Proteins - metabolism / cytoskeleton / Cytoskeleton - physiology / Cytoskeleton - ultrastructure / Epithelial cells / ESTRUCTURA CELULAR / Focal adhesions / genetics / membrane proteins / metabolic inhibitors / metabolism / Mice / Microfilaments / Microscopy, Fluorescence / Oncogene Proteins, Viral / Oncogene Proteins, Viral - genetics / Oncogene Proteins, Viral - metabolism / Papillomaviridae / PAPILLOMAVIRUS BOVIN / Paxillin / pharmacology / Phosphoproteins / Phosphoproteins - metabolism / Phosphorylation / physiology / plasma membrane / Protein Binding / PROTEINAS / PROTEINAS AGLUTINANTES / PROTEINE / PROTEINE DE LIAISON / Proteins / Signal Transduction / STRUCTURE CELLULAIRE / ultrastructure / viral proteins / VIRUS DE LOS ANIMALES / VIRUS DEL PAPILOMA BOVINO / VIRUS DES ANIMAUX / Viruses