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Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice

by Noel, Agnès , Foidart, Jean Michel , Lambert, Vincent , Struman, Ingrid , de Tullio, Pascal , Hansen, Sylvain , Sounni, Nor Eddine , Lecomte, Julie , Blacher, Silvia , Gonzalez, Maria-Luz Alvarez , Rakic, Jean-Marie , Rozet, Eric

in 631/1647/334/1874/345 / 631/1647/767/1657 / 631/378/1689/1626 / 631/443/592/16 / Age Factors / Analytical Chemistry / Animals / Biological Techniques / Bone marrow / Care and treatment / Chemokines / Choroid - pathology / Computational Biology/Bioinformatics / Disease Models, Animal / Drinking water / Drugs / Genetic engineering / Health aspects / Human health sciences / Lasers / Lasers in medicine / Lesions / Life Sciences / Macular degeneration / Macular Degeneration - pathology / Mice / Mice, Inbred C57BL / Microarrays / Neovascularization / Neovascularization, Pathologic / Ophtalmologie / Ophthalmology / Organic Chemistry / Proteins / protocol / Sciences de la santé humaine / Transgenic animals / Vascular endothelial growth factor

2013

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Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice

2013

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Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice

2013

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Journal Article

Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice

Noel, Agnès,

Foidart, Jean Michel,

Lambert, Vincent,

Struman, Ingrid,

de Tullio, Pascal,

Hansen, Sylvain,

Sounni, Nor Eddine,

Lecomte, Julie,

Blacher, Silvia,

Gonzalez, Maria-Luz Alvarez,

Rakic, Jean-Marie,

Rozet, Eric

2013

Overview

The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7–14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.

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Publisher

Nature Publishing Group UK,Nature Publishing Group

Subject

631/1647/334/1874/345

/ Analytical Chemistry

/ Animals