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A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission
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A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission
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Journal Article
A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission
2020
Overview
Ixodes ricinus
is the vector for
Borrelia afzelii
, the predominant cause of Lyme borreliosis in Europe, whereas
Ixodes scapularis
is the vector for
Borrelia burgdorferi
in the USA. Transcription of several
I. scapularis
genes changes in the presence of
B. burgdorferi
and contributes to successful infection. To what extend
B. afzelii
influences gene expression in
I. ricinus
salivary glands is largely unknown. Therefore, we measured expression of uninfected vs. infected tick salivary gland genes during tick feeding using Massive Analysis of cDNA Ends (MACE) and RNAseq, quantifying 26.179 unique transcripts. While tick feeding was the main differentiator,
B. afzelii
infection significantly affected expression of hundreds of transcripts, including 465 transcripts after 24 h of tick feeding. Validation of the top-20
B. afzelii
-upregulated transcripts at 24 h of tick feeding in ten biological genetic distinct replicates showed that expression varied extensively. Three transcripts could be validated, a basic tail protein, a lipocalin and an ixodegrin, and might be involved in
B. afzelii
transmission. However, vaccination with recombinant forms of these proteins only marginally altered
B. afzelii
infection in
I. ricinus
-challenged mice for one of the proteins. Collectively, our data show that identification of tick salivary genes upregulated in the presence of pathogens could serve to identify potential pathogen-blocking vaccine candidates.
Publisher
Nature Publishing Group UK
Subject
/ Animals
/ Arachnid Vectors - microbiology
/ Arthropod Proteins - genetics
/ Bacterial Vaccines - administration & dosage
/ Borrelia burgdorferi Group - drug effects
/ Female
/ Humanities and Social Sciences
/ Lyme Disease - prevention & control
/ Mice
/ Salivary Glands - microbiology
/ Science
/ Tick Infestations - genetics
/ Tick Infestations - microbiology
/ Tick Infestations - prevention & control
