Journal Article

Comparative transcriptomics and proteomics analysis of citrus fruit, to improve understanding of the effect of low temperature on maintaining fruit quality during lengthy post-harvest storage

Ding, Yuduan, Pan, Zhiyong, Deng, Xiuxin, Yun, Ze, Xu, Juan, Wang, Zhuang, Gao, Huijun, Jin, Shuai, Cheng, Yunjiang

2012

Overview

Fruit quality is a very complex trait that is affected by both genetic and non-genetic factors. Generally, low temperature (LT) is used to delay fruit senescence and maintain fruit quality during post-harvest storage but the molecular mechanisms involved are poorly understood. Hirado Buntan Pummelo (HBP;Citrus grandis×C. paradis) fruit were chosen to explore the mechanisms that maintain citrus fruit quality during lengthy LT storage using transcriptome and proteome studies based on digital gene expression (DGE) profiling and two-dimensional gel electrophoresis (2-DE), respectively. Results showed that LT up-regulated stress-responsive genes, arrested signal transduction, and inhibited primary metabolism, secondary metabolism and the transportation of metabolites. Calcineurin B-like protein (CBL)–CBL-interacting protein kinase complexes might be involved in the signal transduction of LT stress, and fruit quality is likely to be regulated by sugar-mediated auxin and abscisic acid (ABA) signalling. Furthermore, ABA was specific to the regulation of citrus fruit senescence and was not involved in the LT stress response. In addition, the accumulation of limonin, nomilin, methanol, and aldehyde, together with the upregulated heat shock proteins, COR15, and cold response-related genes, provided a comprehensive proteomics and transcriptomics view on the coordination of fruit LT stress responses.

• Publisher: Oxford University Press
• Subject: abscisic acid / Abscisic Acid - metabolism / Abscisic Acid - pharmacology / Agriculture / Agronomy. Soil science and plant productions / aldehydes / Anaerobiosis / Anaerobiosis - drug effects / Anaerobiosis - genetics / analysis / Auxins / Biological and medical sciences / Carbohydrates / Carbohydrates - analysis / Carbohydrates - pharmacology / Carboxylic Acids / Carboxylic Acids - metabolism / Cellular senescence / Citrus / Citrus - genetics / Citrus - growth & development / Citrus - metabolism / Citrus - physiology / Citrus maxima / cold / Cold Temperature / drug effects / Electrophoresis, Gel, Two-Dimensional / fruit quality / Fruits / Fundamental and applied biological sciences. Psychology / Gels / gene expression / Gene Expression Profiling / Gene Expression Profiling - methods / Gene expression regulation / Gene Expression Regulation, Plant / Gene Expression Regulation, Plant - drug effects / General agronomy. Plant production / Genes / Genes, Plant / Genes, Plant - genetics / genetics / growth & development / Harvesting. Postharvest. Storage / heat shock proteins / Indoleacetic Acids / Indoleacetic Acids - pharmacology / limonin / metabolism / metabolites / methanol / methods / pharmacology / physiology / Plant physiology and development / Plant Proteins / Plant Proteins - genetics / Plant Proteins - metabolism / Plants / Postharvest handling / Preservation, Biological / Protein metabolism / Proteins / proteome / proteomics / Proteomics - methods / pummelos / RESEARCH PAPER / Research Papers / RNA, Messenger / RNA, Messenger - genetics / RNA, Messenger - metabolism / Senescence and abscission / signal transduction / Signal Transduction - drug effects / Signal Transduction - genetics / Solubility / Solubility - drug effects / stress response / Stress, Physiological / Stress, Physiological - drug effects / Stress, Physiological - genetics / temperature / Time Factors / transcriptome / Transcriptome - drug effects / Transcriptome - genetics / transcriptomics / transportation / two-dimensional gel electrophoresis / Vegetative apparatus, growth and morphogenesis. Senescence / Vegetative propagation. Sowing and planting. Harvesting