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Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
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Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
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Journal Article
Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
2016
Overview
Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid
Escherichia coli
cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system adapts to global perturbations such as nutrient and temperature changes. We demonstrate the efficacy and potential utility of our approach by placing a key metabolic enzyme under optogenetic control, thus enabling dynamic regulation of the culture growth rate with potential applications in bacterial physiology studies and biotechnology.
Optogenetics has emerged as a promising means to achieve gene expression control in bioprocess engineering, but current systems cannot respond to fluctuations in growth conditions. Here the authors overcome this limitation and develop an automated optogenetic feedback control system for precise and robust control of protein production in
E. coli
.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Automation, Laboratory - instrumentation
/ Automation, Laboratory - methods
/ Biotechnology - instrumentation
/ Cell Culture Techniques - instrumentation
/ Cell Culture Techniques - methods
/ E coli
/ Escherichia coli - physiology
/ Escherichia coli Proteins - genetics
/ Escherichia coli Proteins - metabolism
/ Feedback
/ Humanities and Social Sciences
/ Kinases
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Optogenetics - instrumentation
/ Proteins
/ Science
