Journal Article

Evolution of the conformational dynamics of the molecular chaperone Hsp90

Bilgen, Ecenaz, Jussupow, Alexander, Kaila, Ville R. I., Riedl, Stefan, Hirvonen, Viivi, Riedl, Maximilian, Maier, Andreas, Lamb, Don C., Becker, Christian F. W., Tippl, Franziska, Buchner, Johannes, Agam, Ganesh

2024

Overview

Hsp90 is a molecular chaperone of central importance for protein homeostasis in the cytosol of eukaryotic cells, with key functional and structural traits conserved from yeast to man. During evolution, Hsp90 has gained additional functional importance, leading to an increased number of interacting co-chaperones and client proteins. Here, we show that the overall conformational transitions coupled to the ATPase cycle of Hsp90 are conserved from yeast to humans, but cycle timing as well as the dynamics are significantly altered. In contrast to yeast Hsp90, the human Hsp90 is characterized by broad ensembles of conformational states, irrespective of the absence or presence of ATP. The differences in the ATPase rate and conformational transitions between yeast and human Hsp90 are based on two residues in otherwise conserved structural elements that are involved in triggering structural changes in response to ATP binding. The exchange of these two mutations allows swapping of the ATPase rate and of the conformational transitions between human and yeast Hsp90. Our combined results show that Hsp90 evolved to a protein with increased conformational dynamics that populates ensembles of different states with strong preferences for the N-terminally open, client-accepting states. Hsp90 is a molecular chaperone important for protein homeostasis. Here, the authors show that the conformational transitions during the Hsp90 ATPase cycle are conserved from yeast to humans, but a few mutations alter cycle timing and dynamics.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/337/470/1981 / 631/45/470/1981 / 631/45/612/1981 / 631/57/2272/2273 / 631/80/470 / Adenosine triphosphatase / Adenosine Triphosphatases - chemistry / Adenosine Triphosphatases - genetics / Adenosine Triphosphatases - metabolism / Adenosine Triphosphate - metabolism / Cytosol / Dynamics / Evolution, Molecular / Homeostasis / HSP90 Heat-Shock Proteins - chemistry / HSP90 Heat-Shock Proteins - genetics / HSP90 Heat-Shock Proteins - metabolism / Hsp90 protein / Humanities and Social Sciences / Humans / Models, Molecular / multidisciplinary / Mutation / Protein Binding / Protein Conformation / Proteins / Saccharomyces cerevisiae - genetics / Saccharomyces cerevisiae - metabolism / Saccharomyces cerevisiae Proteins - chemistry / Saccharomyces cerevisiae Proteins - genetics / Saccharomyces cerevisiae Proteins - metabolism / Science / Science (multidisciplinary) / Structural members / Structure-function relationships / Yeast