Journal Article

C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes

Awakawa, Takayoshi, Kobayashi, Michihiko, Senda, Toshiya, Abe, Ikuro, He, Haibing, Adachi, Naruhiko, Senda, Miki, Mori, Takahiro, Kumano, Takuto, Watanabe, Satomi, Hori, Sanae, Moriya, Toshio, Hashimoto, Yoshiteru, Terashita, Yuzu, Kawasaki, Masato

2021

Overview

C -Glycosides, in which a sugar moiety is linked via a carbon-carbon (C-C) bond to a non-sugar moiety (aglycone), are found in our food and medicine. The C-C bond is cleaved by intestinal microbes and the resulting aglycones exert various bioactivities. Although the enzymes responsible for the reactions have been identified, their catalytic mechanisms and the generality of the reactions in nature remain to be explored. Here, we present the identification and structural basis for the activation of xenobiotic C -glycosides by heterocomplex C -deglycosylation enzymes from intestinal and soil bacteria. They are found to be metal-dependent enzymes exhibiting broad substrate specificity toward C -glycosides. X-ray crystallographic and cryo-electron microscopic analyses, as well as structure-based mutagenesis, reveal the structural details of these enzymes and the detailed catalytic mechanisms of their remarkable C-C bond cleavage reactions. Furthermore, bioinformatic and biochemical analyses suggest that the C -deglycosylation enzymes are widely distributed in the gut, soil, and marine bacteria. In C-glycosides the sugar moiety is linked through a carbon-carbon bond to the non-sugar moiety, which can be cleaved by intestinal microbes. Here, the authors use bioinformatics analysis to identify C-glycoside deglycosidase enzymes in intestinal and soil bacteria, biochemically characterise them and determine their structures and probe catalytic important residues in mutagenesis experiments.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/326/41/2536 / 631/45/173 / 631/535/1266 / 631/92/173 / 82/80 / 82/83 / Aglycones / Amino Acid Sequence / Bacteria / Bacteria - enzymology / Bacteria - genetics / Bacteria - isolation & purification / Bacterial Proteins - chemistry / Bacterial Proteins - metabolism / Bioinformatics / Carbon / Chemical bonds / Crystallography / Crystallography, X-Ray / Deglycosylation / Digestive system / Enzymes / Gastrointestinal tract / Gastrointestinal Tract - metabolism / Gastrointestinal Tract - microbiology / Glycosides / Glycosides - chemistry / Glycosides - metabolism / Glycosylation / Humanities and Social Sciences / Intestine / Microorganisms / multidisciplinary / Mutagenesis / Phylogeny / Protein Structural Elements / Science / Science (multidisciplinary) / Sequence Homology / Soil bacteria / Soil microorganisms / Soils / Structural analysis / Substrate Specificity / Substrates / Sugar