Journal Article

Fabrication of methylene blue-loaded ovalbumin/polypyrrole nanoparticles for enhanced phototherapy-triggered antitumour immune activation

Zou, Shengqiang, Xu, Xiao, Wu, Yunchao, Du, Fengyi, Yang, Mengyu, Liu, Jingjin, Liu, Suwan, Mao, Huafen, Zhu, Jinqian, Li, Qianzhe

2022

Overview

Background Phototherapy-triggered immunogenic cell death (ICD) rarely elicits a robust antitumour immune response, partially due to low antigen exposure and inefficient antigen presentation. To address these issues, we developed novel methylene blue-loaded ovalbumin/polypyrrole nanoparticles (MB@OVA/PPY NPs) via oxidative polymerization and π–π stacking interactions. Results The as-prepared MB@OVA/PPY NPs with outstanding photothermal conversion efficiency (38%) and photodynamic properties were readily internalized into the cytoplasm and accumulated in the lysosomes and mitochondria. Upon 808 nm and 660 nm laser irradiation, the MB@OVA/PPY NPs not only ablated tumour cells by inducing local hyperthermia but also damaged residual tumour cells by generating a large amount of reactive oxygen species (ROS), finally triggering the release of many damage-associated molecular patterns (DAMPs). Moreover, the MB@OVA/PPY NPs synergized with DAMPs to promote the maturation and improve the antigen presentation ability of DCs in vitro and in vivo. Conclusions This work reported a PPY NPs-based nanoplatform to encapsulate the therepeutic proteins and absorb the functional molecules for combination therapy of tumours. The results demonstrated that the prepared MB@OVA/PPY NPs could be used as effective nanotherapeutic agents to eliminate solid tumours and trigger a powerful antitumour immune response.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Ablation / Analysis / Antigen presentation / Antigens / Apoptosis / Aqueous solutions / Biocompatibility / Biotechnology / Cell activation / Cell death / Chemistry / Chemistry and Materials Science / Cytoplasm / Cytotoxicity / Damage patterns / Fabrication / Fourier transforms / Health aspects / Hemodialysis / Hyperthermia / Immune response / Immune system / Immunogenicity / Immunotherapy / Irradiation / Laser therapy / Lasers / Light therapy / Lysosomes / Methods / Methylene blue / Mitochondria / Molecular Medicine / Nanoparticles / Nanotechnology / Ovalbumin / Particle size / Patient outcomes / Phototherapy / Photothermal conversion / Polypyrrole nanoparticles / Polypyrroles / Polyvinyl alcohol / Reactive oxygen species / Solid tumors / Spectrum analysis / Tumors