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Genome-scale metabolic reconstruction of the symbiosis between a leguminous plant and a nitrogen-fixing bacterium
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Genome-scale metabolic reconstruction of the symbiosis between a leguminous plant and a nitrogen-fixing bacterium
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Journal Article
Genome-scale metabolic reconstruction of the symbiosis between a leguminous plant and a nitrogen-fixing bacterium
2020
Overview
The mutualistic association between leguminous plants and endosymbiotic rhizobial bacteria is a paradigmatic example of a symbiosis driven by metabolic exchanges. Here, we report the reconstruction and modelling of a genome-scale metabolic network of
Medicago truncatula
(plant) nodulated by
Sinorhizobium meliloti
(bacterium). The reconstructed nodule tissue contains five spatially distinct developmental zones and encompasses the metabolism of both the plant and the bacterium. Flux balance analysis (FBA) suggests that the metabolic costs associated with symbiotic nitrogen fixation are primarily related to supporting nitrogenase activity, and increasing N
2
-fixation efficiency is associated with diminishing returns in terms of plant growth. Our analyses support that differentiating bacteroids have access to sugars as major carbon sources, ammonium is the main nitrogen export product of N
2
-fixing bacteria, and N
2
fixation depends on proton transfer from the plant cytoplasm to the bacteria through acidification of the peribacteroid space. We expect that our model, called ‘Virtual Nodule Environment’ (ViNE), will contribute to a better understanding of the functioning of legume nodules, and may guide experimental studies and engineering of symbiotic nitrogen fixation.
The association between leguminous plants and rhizobial bacteria is a paradigmatic example of a symbiosis driven by metabolic exchanges. Here, diCenzo et al. report the reconstruction and modelling of a genome-scale metabolic network of the plant
Medicago truncatula
nodulated by the bacterium
Sinorhizobium meliloti
.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Alfalfa
/ Ammonium
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Genomes
/ Humanities and Social Sciences
/ Legumes
/ Medicago truncatula - genetics
/ Medicago truncatula - metabolism
/ Medicago truncatula - microbiology
/ Mutation
/ Nitrogen
/ Nodules
/ Root Nodules, Plant - growth & development
/ Root Nodules, Plant - metabolism
/ Root Nodules, Plant - microbiology
/ Science
/ Sinorhizobium meliloti - physiology
/ Sugar
