Journal Article

Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein

Gamblin, Steve J., Calvaresi, Valeria, Reading, Eamonn, Roustan, Chloë, Toporowska, Joanna, Wrobel, Antoni G., Hammerschmid, Dietmar, Benton, Donald J., Malim, Michael H., Bradshaw, Richard T., Politis, Argyris, Parsons, Ricardo B., Doores, Katie J.

2023

Overview

SARS-CoV-2 spike glycoprotein mediates receptor binding and subsequent membrane fusion. It exists in a range of conformations, including a closed state unable to bind the ACE2 receptor, and an open state that does so but displays more exposed antigenic surface. Spikes of variants of concern (VOCs) acquired amino acid changes linked to increased virulence and immune evasion. Here, using HDX-MS, we identified changes in spike dynamics that we associate with the transition from closed to open conformations, to ACE2 binding, and to specific mutations in VOCs. We show that the RBD-associated subdomain plays a role in spike opening, whereas the NTD acts as a hotspot of conformational divergence of VOC spikes driving immune evasion. Alpha, beta and delta spikes assume predominantly open conformations and ACE2 binding increases the dynamics of their core helices, priming spikes for fusion. Conversely, substitutions in omicron spike lead to predominantly closed conformations, presumably enabling it to escape antibodies. At the same time, its core helices show characteristics of being pre-primed for fusion even in the absence of ACE2. These data inform on SARS-CoV-2 evolution and omicron variant emergence. In this paper, the authors use hydrogen-deuterium exchange mass spectrometry to describe how the SARS-CoV-2 spike glycoprotein has evolved its structural dynamics features and receptor binding capability from the emergence of the original Wuhan isolate to the recent omicron variant. The findings reported shed light on the evolution of SARS-CoV-2 in the human population and the mechanisms of emergence of new variants.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/1647/296 / 631/326/596/4130 / 631/535 / 631/57/2272 / 82/58 / ACE2 / Amino acid sequence / Amino acids / Angiotensin-Converting Enzyme 2 / Antibodies / Antigens / Binding / COVID-19 / Deuterium / Divergence / Dynamics / Evolution / Glycoproteins / Helices / Human populations / Humanities and Social Sciences / Humans / Hydrogen-deuterium exchange / Mass spectrometry / Mass spectroscopy / Membrane fusion / multidisciplinary / Mutation / Priming / Receptors / SARS-CoV-2 - genetics / Science / Science (multidisciplinary) / Severe acute respiratory syndrome coronavirus 2 / Spike glycoprotein / Spike Glycoprotein, Coronavirus - genetics / Virulence