Journal Article

Unravelling Faecal Microbiota Variations in Equine Atypical Myopathy: Correlation with Blood Markers and Contribution of Microbiome

Lecoq, Laureline, Wouters, Clovis P., van Loon, Gunther, Cesarini, Carla, Palmers, Katrien, Renaud, Benoît, Gustin, Pascal, Votion, Dominique-Marie, François, Anne-Christine, Kruse, Caroline-Julia, Daube, Georges, Taminiau, Bernard, Boemer, François

2025

Overview

Hypoglycin A and methylenecyclopropylglycine are protoxins responsible for atypical myopathy in equids. These protoxins are converted into toxins that inhibit fatty acid β-oxidation, leading to blood accumulation of acylcarnitines and toxin conjugates, such as methylenecyclopropylacetyl-carnitine. The enzymes involved in this activation are also present in some prokaryotic cells, raising questions about the potential role of intestinal microbiota in the development of intoxication. Differences have been noted between the faecal microbiota of cograzers and atypical myopathy-affected horses. However, recent blood acylcarnitines profiling revealed subclinical cases among cograzers, challenging their status as a control group. This study investigates the faecal microbiota of horses clinically affected by atypical myopathy, their cograzers, and a control group of toxin-free horses while analysing correlations between microbiota composition and blood parameters. Faecal samples were analysed using 16S amplicon sequencing, revealing significant differences in α-diversity, evenness, and β-diversity. Notable differences were found between several genera, especially Clostridia_ge, Bacteria_ge, Firmicutes_ge, Fibrobacter, and NK4A214_group. Blood levels of methylenecyclopropylacetyl-carnitine and C14:1 correlated with variations in faecal microbial composition. The theoretical presence of enzymes in bacterial populations was also investigated. These results underscore the critical need to investigate the potential role of intestinal microbiota in this poisoning and may provide insights for developing prevention and treatment strategies.

• Publisher: MDPI AG,MDPI
• Subject: 16S rRNA gene sequencing / Acer / Acer pseudoplatanus / Acylcarnitines / Amino acids / Analysis / Atypical myopathy / Bacteria / Blood / Blood metabolites / Carbohydrates / Chevaux / Enzymes / Equine / equine atypical myopathy / Faecal microbiome / Faecal microbiota / Fatty acids / Gut microbiota / Horses / Hypoglycin A / Hypoglycine A / Hypotheses / Intoxication / Kinases / Life sciences / Maple / Medical examination / Metabolism / Metabolites / Microbiome / Microbiome fécal / Microbiota / Microbiota (Symbiotic organisms) / Microbiote fécal / Microbiote intestinal / Myopathie atypique / Médecine vétérinaire & santé animale / Métabolites sanguins / next generation sequencing / NGS / Pasture / Physiological aspects / Poisoning / Prevention / Prévention / Pâture / Ruminants / Sciences du vivant / Toxicologie / Toxicology Microbiote / Toxin / Toxine / Toxins / Veterinary medicine & animal health / Équins / Érable