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A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression
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A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression
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A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression
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Journal Article
A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression
2018
Overview
Background
The tetracycline-responsive system (Tet-ON/OFF) has proven to be a valuable tool for manipulating gene expression in an inducible, temporal, and tissue-specific manner. The purpose of this study was to create and characterize a new transgenic mouse strain utilizing the human skeletal muscle α-actin (HSA) promoter to drive skeletal muscle-specific expression of the reverse tetracycline transactivator (rtTA) gene which we have designated as the HSA-rtTA mouse.
Methods
To confirm the HSA-rtTA mouse was capable of driving skeletal muscle-specific expression, we crossed the HSA-rtTA mouse with the tetracycline-responsive histone H2B-green fluorescent protein (H2B-GFP) transgenic mouse in order to label myonuclei.
Results
Reverse transcription-PCR confirmed skeletal muscle-specific expression of rtTA mRNA, while single-fiber analysis showed highly effective GFP labeling of myonuclei in both fast- and slow-twitch skeletal muscles. Pax7 immunohistochemistry of skeletal muscle cross-sections revealed no appreciable GFP expression in satellite cells.
Conclusions
The HSA-rtTA transgenic mouse allows for robust, specific, and inducible gene expression across muscles of different fiber types. The HSA-rtTA mouse provides a powerful tool to manipulate gene expression in skeletal muscle.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Biomedical and Life Sciences
/ Cloning
/ Genes
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Humans
/ Kinases
/ Mice
/ Muscle, Skeletal - metabolism
/ Muscle, Skeletal - pathology
/ Muscles
/ PAX7 Transcription Factor - genetics
/ PAX7 Transcription Factor - metabolism
/ RNA
/ Rodents
