Journal Article

NF1 mutation drives neuronal activity-dependent initiation of optic glioma

Chang, Kun-Che, Yalçın, Belgin, Lennon, James J., Cobb, Olivia, Ponnuswami, Anitha, Goldberg, Jeffrey L., Gutmann, David H., Xia, Xin, Schindler, Nicki F., Gibson, Erin M., Monje, Michelle, Ma, Yu, Guo, Xiaofan, Mulinyawe, Sara B., Scheaffer, Suzanne, Liau, Linda M., Huguenard, John R., Pan, Yuan, Hysinger, Jared D., Anastasaki, Corina, Toonen, Joseph A., Barron, Tara

2021

Overview

Neurons have recently emerged as essential cellular constituents of the tumour microenvironment, and their activity has been shown to increase the growth of a diverse number of solid tumours 1 . Although the role of neurons in tumour progression has previously been demonstrated 2 , the importance of neuronal activity to tumour initiation is less clear—particularly in the setting of cancer predisposition syndromes. Fifteen per cent of individuals with the neurofibromatosis 1 (NF1) cancer predisposition syndrome (in which tumours arise in close association with nerves) develop low-grade neoplasms of the optic pathway (known as optic pathway gliomas (OPGs)) during early childhood 3 , 4 , raising  the possibility that postnatal light-induced activity of the optic nerve drives tumour initiation. Here we use an authenticated mouse model of OPG driven by mutations in the neurofibromatosis 1 tumour suppressor gene ( Nf1 ) 5 to demonstrate that stimulation of optic nerve activity increases optic glioma growth, and that decreasing visual experience via light deprivation prevents tumour formation and maintenance. We show that the initiation of Nf1- driven OPGs ( Nf1- OPGs) depends on visual experience during a developmental period in which Nf1 -mutant mice are susceptible to tumorigenesis. Germline Nf1 mutation in retinal neurons results in aberrantly increased shedding of neuroligin 3 (NLGN3) within the optic nerve in response to retinal neuronal activity. Moreover, genetic Nlgn3 loss or pharmacological inhibition of NLGN3 shedding blocks the formation and progression of Nf1- OPGs. Collectively, our studies establish an obligate role for neuronal activity in the development of some types of brain tumours, elucidate a therapeutic strategy to reduce OPG incidence or mitigate tumour progression, and underscore the role of Nf1 mutation-mediated dysregulation of neuronal signalling pathways in mouse models of the NF1 cancer predisposition syndrome. Mouse models of NF1-associated optic pathway glioma show that tumour initiation and growth are driven by aberrantly high levels of NLGN3 shedding in the optic nerve in response to retinal neuron activity.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14 / 14/63 / 38 / 38/91 / 631/378/1689/1690 / 631/67/1922 / 64/110 / 82/51 / 82/58 / Age / Animal models / Animals / Astrocytoma - genetics / Astrocytoma - pathology / Brain cancer / Brain tumors / Cancer / Cell Adhesion Molecules, Neuronal - deficiency / Cell Adhesion Molecules, Neuronal - genetics / Cell Adhesion Molecules, Neuronal - metabolism / Cell Transformation, Neoplastic - genetics / Cell Transformation, Neoplastic - radiation effects / Circadian rhythm / Deprivation / Development and progression / Female / Genes, Neurofibromatosis 1 / Genetic aspects / Genetic disorders / Genetic engineering / Germ-Line Mutation / Glioma / Gliomas / Health aspects / Humanities and Social Sciences / Humans / Light effects / Male / Membrane Proteins - deficiency / Membrane Proteins - genetics / Membrane Proteins - metabolism / Mice / Microenvironments / multidisciplinary / Mutation / Mutation (Biology) / Neoplasia / Neoplasms / Nerve Tissue Proteins - deficiency / Nerve Tissue Proteins - genetics / Nerve Tissue Proteins - metabolism / Nerves / Neurofibromatosis / Neurofibromin 1 - genetics / Neurological disorders / Neurons / Neurons - metabolism / Neurons - radiation effects / Optic nerve / Optic Nerve - cytology / Optic Nerve - radiation effects / Optic Nerve Glioma - genetics / Optic Nerve Glioma - pathology / Photic Stimulation / Physiological aspects / Recklinghausen's disease / Retina / Retina - cytology / Retina - radiation effects / Science / Science (multidisciplinary) / Shedding / Signal transduction / Tumor microenvironment / Tumor suppressor genes / Tumorigenesis / Tumors