Journal Article

Evaluation of Bacillus aryabhattai B8W22 peroxidase for phenol removal in waste water effluents

Hassan, Mervat G., Allam, Nanis G., Shalaby, Azza, El-Said, Karim Samy, Albalawi, Aisha Nawaf, Elmetwalli, Alaa, Salama, Afrah Fatthi

2023

Overview

Environmental contamination by phenol has been reported in both aquatic and atmospheric environments. This study aimed to separate and purify the peroxidase enzyme from bacteria that degrade phenol from wastewater sources. An enrichment culture of MSM was used to screen 25 bacterial isolates from different water samples for peroxidase production, six of the isolates exhibited high levels of peroxidase enzyme activity. Qualitative analysis of peroxidase revealed that isolate No. 4 had the highest halo zones (Poly-R478: 14.79 ± 0.78 mm, Azure B: 8.81 ± 0.61 mm). The promising isolate was identified as Bacillus aryabhattai B8W22 by 16S rRNA gene sequencing with accession number OP458197. As carbon and nitrogen sources, mannitol and sodium nitrate were utilized to achieve maximum peroxidase production. A 30-h incubation period was used with pH 6.0, 30 °C, mannitol, and sodium nitrate, respectively, for maximal production of peroxidase. Purified peroxidase enzyme showed 0.012 U/mg specific activity, and SDS-PAGE analysis indicated a molecular weight of 66 kDa. The purified enzyme exhibits maximum activity and thermal stability at pH values of 4.0 and 8.0, respectively, with maximum activity at 30 °C and complete thermal stability at 40 °C. In the purified enzyme, the Km value was 6.942 mg/ml and the Vmax value was 4.132 mol/ml/hr, respectively. The results demonstrated that Bacillus aryabhattai B8W22 has promising potential for degrading phenols from various phenol-polluted wastewater sources.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Aquatic environment / Bacillus (Bacteria) / Bacillus aryabhattai / Bacillus aryabhattai B8W22 / Bacteria / Biological Microscopy / Biomedical and Life Sciences / Carbon / Carbon sources / Carcinogens / Chemical properties / Contamination / Decomposition (Chemistry) / Effluents / Enrichment media / Environmental aspects / Enzymatic activity / Enzyme activity / Enzymes / Fungi / Gene sequencing / Hydrogen peroxide / Hydrogen-Ion Concentration / Life Sciences / Mannitol / Methods / Microbial enzymes / Microbiology / Molecular weight / Morphology / Mycology / Nitrogen sources / Optimization / Oxidation / Parasitology / Peroxidase / Peroxidase Enzyme activity / Peroxidases / Phenol / Phenol degradation / Phenols / Phenols - chemistry / Physiological aspects / Pollutants / Pollution sources / Polymerization / Purification / Qualitative analysis / RNA, Ribosomal, 16S - genetics / rRNA 16S / Sewage / Sodium / Sodium nitrate / Sodium nitrates / Thermal stability / Virology / Wastewater / Wastewater pollution / Wastewater treatment / Water analysis / Water pollution / Water sampling