Journal Article

Diversity of Cryptosporidium spp. in wild rodents from the Canary Islands, Spain

Martín-Alonso, Aarón, García-Livia, Katherine, Foronda, Pilar

2020

Overview

Background Cryptosporidium spp. are worldwide protozoan parasites which include species that can lead to cryptosporidiosis in humans. Different animal species can serve as reservoirs and sources of dissemination of the disease, such as rodent species due their potential in transmitting zoonotic pathogens to humans and other animals. In the Canary Islands (Spain), Cryptosporidium parvum and Cryptosporidium hominis have been identified in patients with diarrhea. However, the occurrence of Cryptosporidium spp. in possible reservoirs in this archipelago remains unclear. Considering the zoonotic potential of these protozoans, the aim of the present study was to determine the presence of Cryptosporidium spp. in peridomestic wild rodents and the possible role of these mammals as a source of transmission of these protozoans in Canary Islands. Methods A total of 179 rodents belonging to Rattus rattus and Mus musculus domesticus from four Canary Islands, La Palma, El Hierro, Tenerife and Lanzarote, were analyzed. Feces were screened for Cryptosporidium spp. by nested PCR of the 18S ribosomal RNA fragment and the sequences used for phylogenetic analyses. Results Cryptosporidium spp. were found widely distributed with an overall prevalence of 12.30% in rodents (13.86% for R. rattus and 10.25% for M. m. domesticus ). The overall prevalence by island was 19.60% for Tenerife, 7.14% for La Palma, 5.71% for El Hierro and 0% for Lanzarote. Cryptosporidium tyzzeri , Cryptosporidium meleagridis , Cryptosporidium muris and Cryptosporidium sp. rat genotype I and II/III were successfully identified, in addition to two unidentified Cryptosporidium genotypes. Conclusions This study contributes to the knowledge of the biodiversity and distribution of Cryptosporidium spp. in wild rodents from the Canary Islands, highlighting the presence of three zoonotic species, C. tyzzeri , C. meleagridis and C. muris , being the first detection of these three species in wild rodents in the Canary Islands and the first report of C. meleagridis in R. rattus . Given the results obtained in our study, future studies in non-sampled areas are required to better understand the epidemiology of these protozoans in wild rodents in the archipelago.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Animal species / Animals / Animals, Wild - parasitology / Archipelagoes / Biodiversity / Biomedical and Life Sciences / Biomedicine / Canary Islands / Cryptosporidiosis / Cryptosporidiosis - parasitology / Cryptosporidiosis - transmission / Cryptosporidium / Cryptosporidium - classification / Cryptosporidium - genetics / Cryptosporidium - isolation & purification / Cryptosporidium hominis / Cryptosporidium meleagridis / Cryptosporidium muris / Cryptosporidium parvum / Cryptosporidium parvum - genetics / Cryptosporidium parvum - isolation & purification / Cryptosporidium tyzzeri / Deoxyribonucleic acid / Diarrhea / Disease Reservoirs - parasitology / Disease transmission / DNA / Entomology / Epidemics / Epidemiology / Feces / Feces - parasitology / Gene sequencing / Genetic Variation / genotype / Genotype & phenotype / Genotypes / House mouse / Humans / Identification / Infections / Infectious Diseases / Islands / Mice / Nucleic acids / Nucleotide sequence / Parasites / Parasitology / Pathogens / PCR / Phylogenetics / Phylogeny / polymerase chain reaction / Prevalence / Protozoa / Rats / Rattus rattus / Reservoirs (Water) / Ribosomal RNA / RNA / RNA, Ribosomal, 18S - genetics / Rodentia - parasitology / Rodents / rRNA 18S / Spain / Spain - epidemiology / Species / Tropical Medicine / Veterinary Medicine/Veterinary Science / Virology / Wild rodents / Zoonoses / Zoonoses - parasitology / Zoonoses - transmission