Journal Article

An injectable and thermosensitive hydrogel with nano-aided NIR-II phototherapeutic and chemical effects for periodontal antibacteria and bone regeneration

Han, Wei, Wang, Yanyi, Wei, Zheng, Ran, Jianchuan, Chen, Lin, Zhang, Guorong, Cai, Yu, Wang, Weixiang, Zou, Huihui

2023

Overview

Periodontitis is a common public health problem worldwide and an inflammatory disease with irregular defect of alveolar bone caused by periodontal pathogens. Both antibacterial therapy and bone regeneration are of great importance in the treatment of periodontitis. In this study, injectable and thermosensitive hydrogels with 3D networks were used as carriers for controlled release of osteo-inductive agent (BMP-2) and Near Infrared Region-II (NIR-II) phototherapy agents (T8IC nano-particles). T8IC nano-particles were prepared by reprecipitation and acted as photosensitizer under 808 nm laser irradiation. Besides, we promoted photodynamic therapy (PDT) through adding H 2 O 2 to facilitate the antibacterial effect instead of increasing the temperature of photothermal therapy (PTT). Hydrogel + T8IC + Laser + BMP-2 + H 2 O 2 incorporated with mild PTT (45 °C), enhanced PDT and sustained release of BMP-2. It was present with excellent bactericidal effect, osteogenic induction and biosafety both in vitro and in vivo. Besides, immunohistochemistry staining and micro-CT analyses had confirmed that PTT and PDT could promote bone regeneration through alleviating inflammation state. Altogether, this novel approach with synergistic antibacterial effect, anti-inflammation and bone regeneration has a great potential for the treatment of periodontitis in the future.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Advanced local therapies from nano-engineered implants and biomaterials / Alveolar bone / Analysis / Antibacterial activity / Antiinfectives and antibacterials / Bacteria / Bioengineering / Biosafety / Biotechnology / Bone growth / Bone morphogenetic protein 2 / Bone regeneration / Bones / Care and treatment / Chemical effects / Chemistry / Chemistry and Materials Science / Computed tomography / Controlled release / Gels (Pharmacy) / Gum disease / Hydrogels / Hydrogen peroxide / Immunohistochemistry / Inflammatory diseases / Irradiation / Laser radiation / Lasers / Light / Light therapy / Microscopy / Molecular Medicine / Morphology / Nanoparticles / Nanotechnology / Near infrared radiation / Periodontitis / Photodynamic therapy / Phototherapy / Physiological aspects / Public health / Regeneration / Regeneration (physiology) / Sustained release / Technology application / Testing / Thermal properties / Tissue engineering