Asset Details
Do you wish to reserve the book?
Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq
Do you wish to request the book?
Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq
2017
Overview
This protocol describes how to integrate whole-cell patch-clamp in single neurons from mouse brain tissue slices with single-cell RNA sequencing and morphological recovery.
Neurons exhibit a rich diversity of morphological phenotypes, electrophysiological properties, and gene-expression patterns. Understanding how these different characteristics are interrelated at the single-cell level has been difficult because of the lack of techniques for multimodal profiling of individual cells. We recently developed Patch-seq, a technique that combines whole-cell patch-clamp recording, immunohistochemistry, and single-cell RNA-sequencing (scRNA-seq) to comprehensively profile single neurons from mouse brain slices. Here, we present a detailed step-by-step protocol, including modifications to the patching mechanics and recording procedure, reagents and recipes, procedures for immunohistochemistry, and other tips to assist researchers in obtaining high-quality morphological, electrophysiological, and transcriptomic data from single neurons. Successful implementation of Patch-seq allows researchers to explore the multidimensional phenotypic variability among neurons and to correlate gene expression with phenotype at the level of single cells. The entire procedure can be completed in ∼2 weeks through the combined efforts of a skilled electrophysiologist, molecular biologist, and biostatistician.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 631/114
/ 631/378
/ Animals
/ Computational Biology/Bioinformatics
/ Cytology
/ Electrophysiological Phenomena
/ Gene Expression Profiling - methods
/ Immunohistochemistry - methods
/ Mice
/ Neurons
/ Patch-Clamp Techniques - methods
/ protocol
/ Reagents
/ RNA
/ Rodents
/ Sequence Analysis, RNA - methods
