Journal Article

Comparative proteome and transcriptome analyses suggest the regulation of starch and sucrose metabolism and rubber biosynthesis pathways in the recovery of tapping panel dryness in rubber tree

Hu, Yiyu, Wang, Zhenhui, Liu, Hui, Feng, Chengtian, Yuan, Kun, He, Qiguang

2025

Overview

Background Tapping panel dryness (TPD) in rubber tree has become the most severe restricting factor of natural rubber production. To date, there is no effective measures to prevent and control TPD. Previous studies primarily focused on analyzing the molecular mechanism underlying TPD occurrence. However, there is no research on the molecular mechanism of TPD recovery. Results In this study, the TPD trees were recovered by treatment with TPD rehabilitation nutrient agents that could promote the recovery of latex flow on the tapping panel of TPD trees. The genes and proteins involved in TPD recovery were first identified by employing integrated transcriptomics and proteomics analyses. In total, 2029 differentially expressed genes (DEGs) and 951 differentially expressed proteins (DEPs) were detected in the bark of recovery trees compared to that of TPD trees. Among them, 19 DEPs and 11 DEGs were found to be involved in the starch and sucrose metabolism pathway, suggesting their important roles in regulating the syntheses of sucrose and D-glucose, which were the key precursors of natural rubber biosynthesis. Furthermore, 16 DEPs and 15 DEGs were identified in the rubber biosynthesis pathway. Interestingly, almost all the DEPs and DEGs related to rubber biosynthesis exhibited significantly up-regulated expressions in the recovery trees, indicating that latex biosynthesis were probably markedly enhanced during TPD recovery. Conclusions These results provide new insights into the molecular mechanisms underlying TPD recovery, as well as excellent supplements to the mechanisms of TPD occurrence, which will contribute to the development of more effective agents for the prevention and treatment of TPD in the future.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agriculture / Analysis / Bark / Biomedical and Life Sciences / Biosynthesis / Carbohydrate metabolism / Cell death / Diseases and pests / DNA sequencing / Gene expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genomes / Glucose / Growth / Health aspects / Hevea - genetics / Hevea - metabolism / Hevea brasiliensis / Latex / Latex - biosynthesis / Life Sciences / Metabolism / Molecular modelling / Natural rubber / Nucleotide sequencing / Peptides / Physiology / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Proteins / proteome / Proteome - metabolism / Proteomes / Proteomics / Recovery / Rehabilitation / Rubber / Rubber - metabolism / Rubber biosynthesis / Rubber tree / Rubber trees / Signal transduction / Starch / Starch - metabolism / Starch and sucrose metabolism / Sucrose / Sucrose - metabolism / Tapping panel dryness (TPD) / TPD recovery / Transcriptome / Transcriptomes / Transcriptomics / Tree Biology / Trees