Journal Article

Polystyrene and low-density polyethylene degradation by Bacillus cereus L1 isolated from plastic contaminated wastewater

Nasim, Iqra, Nawaz, Rab, Afzaal, Muhammad, Ali, Sikandar, Rehman, Yasir, Zaki, Magdi E. A., Al-Hussain, Sami A., Iqbal, Syeda Saira, Irfan, Ali

2025

Overview

Background The environmental disruption caused by enduring pollution has been aggravated by the excessive use of low-density polyethylene (LDPE) and polystyrene (PS) plastics due to their non-biodegradable composition. Microbial bioremediation could be an environmentally friendly approach to degrade these pollutants. In this research, plastic-degrading bacteria were isolated from plastic-polluted wastewater collected from a wastewater drain. Methodology For this study, enrichment culture techniques were employed to isolate plastic-degrading bacteria from wastewater contaminated with plastic. Characterization was performed on the purified isolate with the highest efficiency. The bacteria utilizing PS and LDPE as the sole carbon sources were purified and characterized (FTIR, SEM) based on morphology, antibiotic resistance profile, and phylogenetic analysis through 16 S rRNA gene sequencing. Results The bacteria showing the most significant PS and LDPE degradation were identified as B. cereus L1. The strain also exhibited biofilm-forming ability, confirmed by a qualitative crystal violet ring test, a pivotal factor in plastic breakdown.  B. cereus L1 reduced the weight of LDPE beads from 1.08 g to 0.87 g and from 1.15 g to 0.92 g in four weeks. Fourier Transform Infrared Radiation (FTIR) spectroscopy revealed changes in the structural configuration of PS and LDPE after incubation with B. cereus L1, as distinct peaks at 2196 cm⁻¹ for alkynes, nitriles, and 1715 cm⁻¹ for carbonyl groups were observed, indicating the degradation of the polymers. Conclusion This work highlights the potential of B. cereus L1 in bioremediation strategies for managing plastic waste, offering a sustainable and eco-friendly alternative for combating plastic pollution in both terrestrial and aquatic ecosystems.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Alkynes / Antibiotic resistance / Antibiotics / Applied Microbiology / Aquatic ecosystems / Bacillus / Bacillus cereus / Bacteria / Bioaccumulation / Biodegradation / Biofilms / Bioremediation / Biotechnology / Carbon / Carbon sources / Carbonyl compounds / Carbonyl groups / Carbonyls / Chemistry / Chemistry and Materials Science / Culture techniques / Degradation / Density / Drug resistance in microorganisms / Enrichment media / Environmental aspects / Enzymology / Fourier transforms / Gene sequencing / Genetic Engineering / Gentian violet / I.R. radiation / Infrared radiation / Infrared spectroscopy / Low density polyethylenes / Low-Density polyethylene / Methods / Microbial Genetics and Genomics / Microbiology / Microorganisms / Mineralization / Molecular weight / Neomycin / Nitriles / Phylogeny / Physiological aspects / Plastic debris / Plastic degradation / Plastic pollution / Polyethylene / Polymers / Polystyrene / Polystyrene resins / RNA / rRNA / Sodium / Temperature / Tetracycline / Tetracyclines / Waste management / Wastewater / Wastewater pollution