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Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system
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Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system
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Journal Article
Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system
2021
Overview
Background
Vector-borne diseases pose an increasing threat to global food security. Vaccines, diagnostic tests, and therapeutics are urgently needed for tick-borne diseases that affect livestock. However, the inability to obtain significant quantities of pathogen stages derived from ticks has hindered research. In vitro methods to isolate pathogens from infected tick vectors are paramount to advance transcriptomic, proteomic, and biochemical characterizations of tick-borne pathogens.
Methods
Nymphs of
Rhipicephalus appendiculatus
were infected with
Theileria parva
by feeding on a calf during an acute infection. Isolation of sporozoites was accomplished by feeding infected adult ticks on an in vitro tick feeding system. Sporozoite viability was tested using in vitro bovine lymphocytes.
Results
We isolated infectious
T. parva
sporozoites secreted into an in vitro tick feeding system. Infected adult
R. appendiculatus
ticks attached to and successfully fed on silicone membranes in the in vitro tick feeding system. Bovine blood in the receptacle was replaced with cell-free medium and the ticks were allowed to feed for 3 h to collect secreted
T. parva
sporozoites. Secreted sporozoites infected in vitro bovine lymphocytes, demonstrating that isolated sporozoites remained viable and infectious.
Conclusions
This work is the first to report the isolation of mature infectious
T. parva
sporozoites using an in vitro tick feeding system, which represents a significant step towards the development of a more efficient control strategy for
T. parva
. Isolation of infectious tick-stage parasites will facilitate the examination of the vector-pathogen interface, thereby accelerating the development of next-generation vaccines and treatment interventions for tick-borne pathogens.
Graphical Abstract
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Biomedical and Life Sciences
/ blood
/ calves
/ Cattle
/ Control
/ Diseases
/ Feeding
/ Feeds
/ Identification and classification
/ In vitro
/ In vitro tick feeding system
/ Nymphs
/ Protozoa and protozoan diseases
/ Rhipicephalus - parasitology
/ Rhipicephalus appendiculatus
/ silicone
/ Theileria parva - physiology
/ Ticks
/ Vaccines
/ Vectors
/ Veterinary Medicine/Veterinary Science
/ Virology
