Journal Article

Serological and molecular analysis of henipavirus infections in synanthropic fruit bat and rodent populations in the Centre and North regions of Cameroon (2018–2020)

Mbu’u, Cyrille Mbanwi, Wade, Abel, Mbacham, Wilfred Fon, Penning, Maren, Sadeghi, Balal, LeBreton, Matthew, Mbange, Aristid Ekollo, Gontao, Pierre, Balkema-Buschmann, Anne, Kamdem, Sylvain Leroy Sado, Groschup, Martin Hermann, Stoek, Franziska

2025

Overview

Background Bats and rodents have been identified as reservoirs for several highly pathogenic and zoonotic viruses including henipaviruses, a genus within the Paramyxoviridae family. A number of studies have revealed the circulation of henipaviruses at the wildlife-human-livestock interface in Cameroon. In this study, we describe the molecular analysis as well as the development and evaluation of a Bead-based Multiplex Binding Assay (BMBA) using an in-house Indirect Enzyme Linked Immunosorbent Assay (ELISA) to confirm the detection of henipavirus infection in wildlife species. Results A total of 600 fruit bats and 600 rodents were sampled between March 2018 and June 2020. Samples were analyzed using a semi-nested RT-PCR assay followed by sequencing of the PCR fragments. Transudates (754) were screened for the presence of henipavirus-specific antibodies in a BMBA and confirmed by ELISA using Hendra virus (HeV), Nipah virus (NiV) and Ghana virus (GhV) glycoproteins expressed in Leishmania tarentolae , and commercially available HeV G and NiV G glycoproteins. Henipavirus-specific antibodies were detected in 19/531 (3.6%) bat transudates screened by BMBA and confirmed by ELISA. Seroprevalence rates in the Centre and North Regions were 12/291 (4.1%) and 7/240 (2.9%) respectively. All rodents and shrews were serologically negative. Henipavirus RNA sequences were not detected in any of the samples screened in this work. Conclusion This study provides further data supporting the circulation of Henipaviruses in fruit bats ( Eidolon helvum ) which are roosting and reproducing in proximity to human and livestock populations in the Centre and North Regions of Cameroon. This also establishes the first detection of Henipavirus specific antibodies in Eidolon helvum populations in the North Region of Cameroon.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Animals / Antibodies / Antibodies, Viral - blood / Bats / Bead-based multiplex binding assay and ELISA / Cameroon / Cameroon - epidemiology / Centre and North regions of Cameroon / Chiroptera / Chiroptera - virology / Coronaviruses / Disease transmission / Eidolon helvum / Enzyme-linked immunosorbent assay / Enzyme-Linked Immunosorbent Assay - veterinary / enzymes / family / Fatalities / Fruit bats and rodents / Fruits / genus / Ghana / Glycoproteins / Health aspects / Hendra henipavirus / Henipavirus / Henipavirus - genetics / Henipavirus - immunology / Henipavirus - isolation & purification / Henipavirus Infections - epidemiology / Henipavirus Infections - veterinary / Henipavirus Infections - virology / Henipaviruses / Horses / humans / Infections / Leishmania tarentolae / Livestock / Medicine / Medicine & Public Health / Microbial mutation / Nipah henipavirus / Pathogens / Polymerase chain reaction / RNA / Rodent Diseases - epidemiology / Rodent Diseases - virology / Rodentia - virology / Rodents / Seroepidemiologic Studies / Serology / seroprevalence / species / synanthropes / Testing / Transgenics / Veterinary Medicine/Veterinary Science / Viral antibodies / Viruses / Wildlife / Zoology / Zoonoses / Zoonotic disease / Zoonotic diseases