Journal Article

Complete protection of the BALB/c and C57BL/6J mice against Ebola and Marburg virus lethal challenges by pan-filovirus T-cell epigraph vaccine

Hanke, Tomáš, Tierney, Kevin, Baines, Andrea, Qiu, Xiangguo, Moyo, Nathifa, Wee, Edmund G., Hannoun, Zara, Rahim, Md Niaz, He, Shihua, Korber, Bette, Audet, Jonathan, Crook, Alison

2019

Overview

There are a number of vaccine candidates under development against a small number of the most common outbreak filoviruses all employing the virus glycoprotein (GP) as the vaccine immunogen. However, antibodies induced by such GP vaccines are typically autologous and limited to the other members of the same species. In contrast, T-cell vaccines offer a possibility to design a single pan-filovirus vaccine protecting against all known and even likely existing, but as yet unencountered members of the family. Here, we used a cross-filovirus immunogen based on conserved regions of the filovirus nucleoprotein, matrix and polymerase to construct simian adenovirus- and poxvirus MVA-vectored vaccines, and in a proof-of-concept study demonstrated a protection of the BALB/c and C57BL/6J mice against high, lethal challenges with Ebola and Marburg viruses, two distant members of the family, by vaccine-elicited T cells in the absence of GP antibodies.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adenoviruses / Amino acids / Analysis / Animals / Antibodies / Antibodies, Neutralizing / Antibodies, Viral / Antigens / Biology and Life Sciences / Clinical trials / Deoxyribonucleic acid / DNA / Ebola Vaccines / Ebola virus / Ebolavirus / Ebolavirus - pathogenicity / Epidemics / Female / Filoviridae / Filoviridae - immunology / Filoviridae - metabolism / Filoviridae - pathogenicity / Glycoproteins / Hemorrhagic Fever, Ebola / House mouse / Immunity, Cellular - immunology / Infections / Infectious diseases / Laboratories / Lymphocytes / Lymphocytes T / Male / Marburg disease / Marburg virus disease / Marburgvirus / Marburgvirus - pathogenicity / Mathematical analysis / Matrix methods / Medicine / Medicine and Health Sciences / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Monkeys / Outbreaks / Proof of Concept Study / Public health / Research and Analysis Methods / T cells / T-Lymphocytes - immunology / T-Lymphocytes - metabolism / Vaccines / Viral diseases / Viral Vaccines - pharmacology / Viruses