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Effects of preoperative topical levofloxacin on conjunctival microbiome in patients undergoing intravitreal injections
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Effects of preoperative topical levofloxacin on conjunctival microbiome in patients undergoing intravitreal injections
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Effects of preoperative topical levofloxacin on conjunctival microbiome in patients undergoing intravitreal injections
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Journal Article
Effects of preoperative topical levofloxacin on conjunctival microbiome in patients undergoing intravitreal injections
2025
Overview
Endophthalmitis is a serious eye infection that can occur after intravitreal injections. Topical antibiotics are frequently used as a preventative measure, but their impact on the conjunctival microbiome is not fully understood.
Conjunctival swabs were collected from 33 eyes of 33 patients undergoing intravitreal injections, both before and after a 3-day course of prophylactic topical levofloxacin 0.5%. Conjunctival microbiome analysis was conducted using 16S rRNA sequencing on the Illumina MiSeq platform. Bioinformatics processing identified unique amplicon sequence variants (ASVs) to evaluate microbial diversity and community composition. Alpha and beta diversity indices were analyzed.
Topical levofloxacin treatment resulted in no significant change in alpha diversity indices, including Shannon index, Chao1, Shannon, PD whole tree, and observed ASVs, indicating stable microbial richness and evenness. In contrast, beta diversity analysis, assessed through Bray-Curtis dissimilarity, revealed significant differences in microbial composition between pre- and post-treatment samples. These changes included a decrease in the abundance of Staphylococcus and Bacillus species and an increase in the abundance of Streptococcus, Haemophilus, and Neisseria.
Although prophylactic topical levofloxacin was found to alter the conjunctival microbiome and showed inconsistent effects on the abundance of pathogenic bacteria, its clinical effectiveness as a preventative measure against endophthalmitis remains inconclusive. Further studies are needed to clarify its role in infection prevention.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Aged
/ Anti-Bacterial Agents - administration & dosage
/ Anti-Bacterial Agents - pharmacology
/ Anti-Bacterial Agents - therapeutic use
/ Ecology and Environmental Sciences
/ Endophthalmitis - microbiology
/ Endophthalmitis - prevention & control
/ Female
/ Humans
/ Intravitreal Injections - adverse effects
/ Iodine
/ Levofloxacin - administration & dosage
/ Levofloxacin - therapeutic use
/ Male
/ Medicine and Health Sciences
/ Patients
/ RNA, Ribosomal, 16S - genetics
/ rRNA 16S
/ Testing
