Journal Article

Transcriptomic and metabolomic analysis reveal the cold tolerance mechanism of common beans under cold stress

Sui, Xiyu, Sun, Bo, Li, Zixuan, Li, Huanxiu, Tang, Wen, Xu, Zeping, Xiao, Jiachang, Liang, Le, Huang, Zhi, Song, Xueping, Wang, Changquan, Lai, Yunsong, Tang, Yi

2025

Overview

Background Common bean ( Phaseolus vulgaris L.) is a thermophilic crop, and exposure to cold stress can significantly impact their yield and quality. To elucidate the impact of cold stress on cold-tolerant ‘Wei Yuan’ (WY) and cold-sensitive ‘Bai Bu Lao’ (BBL) of common bean, the mechanism of cold tolerance was studied by physiological and biochemical and multi-omics analysis. Results In this study, lower relative conductivity and higher malondialdehyde content after cold stress endowed ‘WY’ seedlings with cold tolerance. A total of 11,837 differentially expressed genes (DEGs) and 923 differential metabolites (DEMs) were identified by transcriptome and metabolomics analysis. Joint analysis showed that under cold stress, DEGs and DEMs in common beans are extensively engaged in sugar, amino acid and isoflavonoid biosynthesis, flavone and flavonol biosynthesis, and plant hormone signal translation, especially related to isoflavone biosynthesis. In addition, it was also found that bHLH and MYB family transcription factors may be involved in the cold signal transduction of common bean. Conclusions The above results will provide a theoretical basis for the cold tolerance mechanism of common beans and provide help for the screening of cold-tolerant resources of common beans. Clinical trial number Not applicable.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agricultural production / Agriculture / Amino acids / Beans / Biomedical and Life Sciences / Biosynthesis / Botanical research / Chemical properties / clinical trials / cold / Cold stress / Cold Temperature / Cold tolerance / Cold weather / Cold-Shock Response - genetics / Common beans / Data analysis / Environmental aspects / Enzymes / family / Flavonols / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genetic aspects / Hardiness / Hormones / Humidity / Isoflavone / isoflavones / Legumes / Life Sciences / Light / malondialdehyde / Metabolism / Metabolites / Metabolome / Metabolomics / Multi-omics / multiomics / Omics technologies and applications in horticultural crops / Phaseolus - genetics / Phaseolus - metabolism / Phaseolus - physiology / Phaseolus vulgaris / Physiological aspects / Physiology / Plant genetics / Plant growth / Plant hormones / Plant metabolites / Plant resistance / Plant Sciences / Plants / Proteins / Secondary metabolites / Seedlings / Seeds / Signal transduction / Stress (Physiology) / sugars / Temperature / Transcription factors / Transcriptome / Transcriptomes / Transcriptomics / Tree Biology