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The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

by Podestà, Alessandro , Previdi, Anita , D’Urso, Mirko , Piazzoni, Claudio , Chighizola, Matteo , Lenardi, Cristina , Schulte, Carsten , Marcotti, Stefania , Stramer, Brian , Folliero, Claudia , Dini, Tania , Milani, Paolo , Borghi, Francesca , Ceriani, Laura

in Actin / Biomedical materials / Biotechnology / Cell research / Cellular signal transduction / Cellular structure / Chemistry / Chemistry and Materials Science / Complexity / Compliance / Configurations / Extracellular matrix / Focal adhesion / Force loading / Glycocalyx / Impact analysis / Integrin adhesion complexes / Integrins / Interfaces / Mechanical properties / Mechanotransduction / Microenvironments / Molecular clutch / Molecular Medicine / Morphology / Nanotechnology / Pheochromocytoma cells / Physiological aspects / Spectroscopy / Substrates

2022

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The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

2022

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2022

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Journal Article

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

Podestà, Alessandro,

Previdi, Anita,

D’Urso, Mirko,

Piazzoni, Claudio,

Chighizola, Matteo,

Lenardi, Cristina,

Schulte, Carsten,

Marcotti, Stefania,

Stramer, Brian,

Folliero, Claudia,

Dini, Tania,

Milani, Paolo,

Borghi, Francesca,

Ceriani, Laura

2022

Overview

The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale ( e.g. , by force spectroscopy with functionalised probes). Our data demonstrates that the glycocalyx configuration affects spatio-temporal nanotopography-sensitive mechanotransductive events at the cell/microenvironment interface. Opposing effects of major glycocalyx removal were observed, when comparing flat and specific nanotopographical conditions. The excessive retrograde actin flow speed and force loading are strongly reduced on certain nanotopographies upon strong reduction of the native glycocalyx, while on the flat substrate we observe the opposite trend. Our results highlight the importance of the glycocalyx configuration in a molecular clutch force loading-dependent cellular mechanism for mechanosensing of microenvironmental nanotopographical features. Graphical Abstract

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Publisher

BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC

Subject

Actin

/ Biomedical materials

/ Biotechnology

/ Cell research

/ Cellular signal transduction

/ Cellular structure

/ Chemistry