Asset Details
Do you wish to reserve the book?
METTL3 regulates heterochromatin in mouse embryonic stem cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
METTL3 regulates heterochromatin in mouse embryonic stem cells
Do you wish to request the book?
METTL3 regulates heterochromatin in mouse embryonic stem cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
METTL3 regulates heterochromatin in mouse embryonic stem cells
2021
Overview
METTL3 (methyltransferase-like 3) mediates the
N
6
-methyladenosine (m
6
A) methylation of mRNA, which affects the stability of mRNA and its translation into protein
1
. METTL3 also binds chromatin
2
–
4
, but the role of METTL3 and m
6
A methylation in chromatin is not fully understood. Here we show that METTL3 regulates mouse embryonic stem-cell heterochromatin, the integrity of which is critical for silencing retroviral elements and for mammalian development
5
. METTL3 predominantly localizes to the intracisternal A particle (IAP)-type family of endogenous retroviruses. Knockout of
Mettl3
impairs the deposition of multiple heterochromatin marks onto METTL3-targeted IAPs, and upregulates IAP transcription, suggesting that METTL3 is important for the integrity of IAP heterochromatin. We provide further evidence that RNA transcripts derived from METTL3-bound IAPs are associated with chromatin and are m
6
A-methylated. These m
6
A-marked transcripts are bound by the m
6
A reader YTHDC1, which interacts with METTL3 and in turn promotes the association of METTL3 with chromatin. METTL3 also interacts physically with the histone 3 lysine 9 (H3K9) tri-methyltransferase SETDB1 and its cofactor TRIM28, and is important for their localization to IAPs. Our findings demonstrate that METTL3-catalysed m
6
A modification of RNA is important for the integrity of IAP heterochromatin in mouse embryonic stem cells, revealing a mechanism of heterochromatin regulation in mammals.
Binding of METTL3 to chromatin is enriched over IAP family endogenous retroviral elements in mouse embryonic stem cells, helping to ensure the integrity of heterochromatin at these elements.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/89
/ 38/22
/ 38/23
/ 38/77
/ 42/70
/ 45
/ 45/90
/ 45/91
/ Animals
/ Chromatin Assembly and Disassembly
/ DNA
/ Endogenous Retroviruses - genetics
/ Genes, Intracisternal A-Particle - genetics
/ Heterochromatin - metabolism
/ Histone-Lysine N-Methyltransferase - metabolism
/ Histones
/ Humanities and Social Sciences
/ Lysine
/ Mammals
/ Methyltransferases - metabolism
/ Mice
/ Mouse Embryonic Stem Cells - metabolism
/ Science
