Journal Article

Transcriptome analysis under pecan scab infection reveals the molecular mechanisms of the defense response in pecans

Pisani, Cristina, Schmutz, Jeremy, Sreedasyam, Avinash, Jenkins, Jerry, Lovell, John T., Rhein, Hormat Shadgou, Grimwood, Jane, Bhattarai, Gaurab, Chee, Peng W., Randall, Jennifer, Conner, Patrick J., Khanal, Sameer

2024

Overview

Pecan scab, caused by the fungal pathogen Venturia effusa , is the most devastating disease of pecan ( Carya illinoinensis ) in the southeastern United States. Resistance to this pathogen is determined by a complex interaction between host genetics and disease pathotype with even field-susceptible cultivars being resistant to most scab isolates. To understand the underlying molecular mechanisms of scab resistance in pecan, we performed a transcriptome analysis of the pecan cultivar, ‘Desirable’, in response to inoculation with a pathogenic and a non-pathogenic scab isolate at three different time points (24, 48, and 96 hrs. post-inoculation). Differential gene expression and gene ontology enrichment analyses showed contrasting gene expression patterns and pathway enrichment in response to the contrasting isolates with varying pathogenicity. The weighted gene co-expression network analysis of differentially expressed genes detected 11 gene modules. Among them, two modules had significant enrichment of genes involved with defense responses. These genes were particularly upregulated in the resistant reaction at the early stage of fungal infection (24 h) compared to the susceptible reaction. Hub genes in these modules were predominantly related to receptor-like protein kinase activity, signal reception, signal transduction, biosynthesis and transport of plant secondary metabolites, and oxidoreductase activity. Results of this study suggest that the early response of pathogen-related signal transduction and development of cellular barriers against the invading fungus are likely defense mechanisms employed by pecan cultivars against non-virulent scab isolates. The transcriptomic data generated here provide the foundation for identifying candidate resistance genes in pecan against V . effusa and for exploring the molecular mechanisms of disease resistance.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Ascomycota - pathogenicity / Ascomycota - physiology / Biology and Life Sciences / Biosynthesis / Carya - genetics / Carya - microbiology / Carya illinoinensis / Cultivars / Defense mechanisms / Disease resistance / Disease Resistance - genetics / Diseases and pests / Enrichment / Fungal diseases of plants / Fungi / Fungicides / Gene expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Gene Ontology / Genes / Genetic aspects / Genetics / Infections / Inoculation / Kinases / Leaves / Medicine and Health Sciences / Metabolites / Modules / Molecular modelling / Network analysis / Oxidoreductase / Pathogenicity / Pathogens / pathotypes / Pecan / Pecan nuts / pecan scab / pecans / Pesticides / Physiological aspects / Plant Diseases - genetics / Plant Diseases - immunology / Plant Diseases - microbiology / Plant immunology / Protein biosynthesis / protein kinases / Scab / Secondary metabolites / Signal reception / Signal transduction / Transcriptome / Transcriptomes / Transcriptomics / Venturia effusa / Virulence