Journal Article

A Phase II Trial of Sorafenib in Metastatic Melanoma with Tissue Correlates

Yoon, Joanne, Pavlick, Anna C., Wright, John J., Hamilton, Anne, Buckley, Michael, Yee, Herman, Christos, Paul J., Goldberg, Lauren, Liebes, Leonard, Osman, Iman, Ott, Patrick A., Safarzadeh-Amiri, Sara, Min, Christina, Polsky, David

2010

Overview

Sorafenib monotherapy in patients with metastatic melanoma was explored in this multi-institutional phase II study. In correlative studies the impact of sorafenib on cyclin D1 and Ki67 was assessed. Thirty-six patients treatment-naïve advanced melanoma patients received sorafenib 400 mg p.o. twice daily continuously. Tumor BRAF(V600E) mutational status was determined by routine DNA sequencing and mutation-specific PCR (MSPCR). Immunohistochemistry (IHC) staining for cyclin D1 and Ki67 was performed on available pre- and post treatment tumor samples. The main toxicities included diarrhea, alopecia, rash, mucositis, nausea, hand-foot syndrome, and intestinal perforation. One patient had a RECIST partial response (PR) lasting 175 days. Three patients experienced stable disease (SD) with a mean duration of 37 weeks. Routine BRAF(V600E) sequencing yielded 27 wild-type (wt) and 6 mutant tumors, whereas MSPCR identified 12 wt and 18 mutant tumors. No correlation was seen between BRAF(V600E) mutational status and clinical activity. No significant changes in expression of cyclin D1 or Ki67 with sorafenib treatment were demonstrable in the 15 patients with pre-and post-treatment tumor samples. Sorafenib monotherapy has limited activity in advanced melanoma patients. BRAF(V600E) mutational status of the tumor was not associated with clinical activity and no significant effect of sorafenib on cyclin D1 or Ki67 was seen, suggesting that sorafenib is not an effective BRAF inhibitor or that additional signaling pathways are equally important in the patients who benefit from sorafenib. Clinical Trials.gov NCT00119249.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adult / Aged / Aged, 80 and over / Alopecia / Antineoplastic Agents - therapeutic use / Apoptosis / Benzenesulfonates - therapeutic use / Biology / Cancer metastasis / Cancer therapies / Cell cycle / Clinical Trial / Clinical trials / Correlation analysis / Cyclin D1 / Cyclin D1 - biosynthesis / Deoxyribonucleic acid / Dermatology / Diarrhea / DNA / DNA Mutational Analysis / DNA sequencing / Female / Gene sequencing / Growth factors / Humans / Immunohistochemistry / Inhibitor drugs / Intestine / Ki-67 Antigen - biosynthesis / Male / Medical research / Medicine / Melanoma / Melanoma - drug therapy / Metastases / Metastasis / Middle Aged / Mucositis / Mutation / Nausea / Neoplasm Metastasis / Niacinamide - analogs & derivatives / Oncology / Patients / Perforation / Phenylurea Compounds / Proto-Oncogene Proteins B-raf - metabolism / Pyridines - therapeutic use / Review boards / Sequence Analysis, DNA / Signal Transduction / Skin cancer / Sorafenib / Targeted cancer therapy / Toxicity / Tumors