Journal Article

The Multifaceted Effects of Polysaccharides Isolated from Dendrobium huoshanense on Immune Functions with the Induction of Interleukin-1 Receptor Antagonist (IL-1ra) in Monocytes

Lin, Juway, Wong, Chi-Huey, Yu, Alice L., Chang, Ya-Jen, Yang, Wen-Bin

2014

Overview

Dendrobium huoshanense is a valuable and versatile Chinese herbal medicine with the anecdotal claims of cancer prevention and anti-inflammation. However, its immunological activities are limited to in vitro studies on a few cytokines and immune cell functions. First, we investigated the effects of polysaccharides isolated from DH (DH-PS) on inducing a panel of cytokines/chemokines in mice in vivo and human in vitro. We found that DH polysaccharides (DH-PS) induced TH1, TH2, inflammatory cytokines and chemokines in mouse in vivo and human cells in vitro. Secondly, we demonstrated that DH-PS expanded mouse splenocytes in vivo including CD4(+) T cells, CD8(+) T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells. Notably, DH-PS induced an anti-inflammatory molecule, IL-1ra, in mouse and human immune cells, especially monocytes. The serum level of IL-1ra elicited by the injection of DH-PS was over 10 folds of IL-1β, suggesting that DH-PS-induced anti-inflammatory activities might over-ride the inflammatory ones mediated by IL-1β. The signaling pathways of DH-PS-induced IL-1ra production was shown to involve ERK/ELK, p38 MAPK, PI3K and NFκB. Finally, we observed that IL-1ra level induced by DH-PS was significantly higher than that by F3, a polysaccharide extract isolated from another popular Chinese herbal medicine, Ganoderma lucidum. These results indicated that DH-PS might have potential applications for ameliorating IL-1-induced pathogenic conditions.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: 1-Phosphatidylinositol 3-kinase / Analysis / Animals / B cells / Binding sites / Biology and Life Sciences / Cancer / CD4 antigen / CD8 antigen / Chemokines / Chemokines - biosynthesis / Cytokines / Cytokines - biosynthesis / Dendrobium / Dendrobium - chemistry / Disease prevention / Dose-Response Relationship, Drug / Drugs, Chinese Herbal - chemistry / Elk / Female / Ganoderma / Ganoderma lucidum / Gene expression / Genomics / Herbal medicine / Humans / Immune system / Immune System Phenomena - drug effects / Immune System Phenomena - physiology / Immunologic Factors - immunology / Immunologic Factors - pharmacology / Immunology / Immunoregulation / Inflammation / Interleukin 1 / Interleukin 1 receptor antagonist / Interleukin 1 Receptor Antagonist Protein - metabolism / Interleukin 1 receptors / Interleukins / Kinases / Leukocytes (granulocytic) / Leukocytes, Mononuclear - drug effects / Leukocytes, Mononuclear - immunology / Leukocytes, Mononuclear - metabolism / Lymphocytes / Lymphocytes B / Lymphocytes T / Macrophages / MAP kinase / Medical research / Medicine / Medicine and Health Sciences / Mice / Monocytes / Monocytes - drug effects / Monocytes - immunology / Monocytes - metabolism / Mushrooms / Natural killer cells / Neutrophils / NF-κB protein / Plant Exudates - pharmacology / Polysaccharides / Polysaccharides - immunology / Polysaccharides - pharmacology / Research and Analysis Methods / Rheumatoid arthritis / Saccharides / Sepsis / Signal transduction / Signal Transduction - drug effects / Signaling / Splenocytes / Studies / T cells