Journal Article

Tumor-Associated Macrophages in Glioblastoma Multiforme—A Suitable Target for Somatostatin Receptor-Based Imaging and Therapy?

Linsenmann, Thomas, Herrmann, Ken, Stoffer, Carolin, Samnick, Samuel, Monoranu, Camelia-Maria, Ernestus, Ralf-Ingo, Lückerath, Katharina, Lapa, Constantin, Buck, Andreas K., Löhr, Mario

2015

Overview

Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults. Tumor-associated macrophages (TAM) have been shown to promote malignant growth and to correlate with poor prognosis. [1,4,7,10-tetraazacyclododecane-NN',N″,N'″-tetraacetic acid]-d-Phe1,Tyr3-octreotate (DOTATATE) labeled with Gallium-68 selectively binds to somatostatin receptor 2A (SSTR2A) which is specifically expressed and up-regulated in activated macrophages. On the other hand, the role of SSTR2A expression on the cell surface of glioma cells has not been fully elucidated yet. The aim of this study was to non-invasively assess SSTR2A expression of both glioma cells as well as macrophages in GBM. 15 samples of patient-derived GBM were stained immunohistochemically for macrophage infiltration (CD68), proliferative activity (Ki67) as well as expression of SSTR2A. Anti-CD45 staining was performed to distinguish between resident microglia and tumor-infiltrating macrophages. In a subcohort, positron emission tomography (PET) imaging using 68Ga-DOTATATE was performed and the semiquantitatively evaluated tracer uptake was compared to the results of immunohistochemistry. The amount of microglia/macrophages ranged from <10% to >50% in the tumor samples with the vast majority being resident microglial cells. A strong SSTR2A immunostaining was observed in endothelial cells of proliferating vessels, in neurons and neuropile. Only faint immunostaining was identified on isolated microglial and tumor cells. Somatostatin receptor imaging revealed areas of increased tracer accumulation in every patient. However, retention of the tracer did not correlate with immunohistochemical staining patterns. SSTR2A seems not to be overexpressed in GBM samples tested, neither on the cell surface of resident microglia or infiltrating macrophages, nor on the surface of tumor cells. These data suggest that somatostatin receptor directed imaging and treatment strategies are less promising in GBM.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adult / Adults / Aged / Aged, 80 and over / Antigens, CD - metabolism / Antigens, Differentiation, Myelomonocytic - metabolism / Brain / Brain cancer / Brain Neoplasms - diagnostic imaging / Brain Neoplasms - drug therapy / Brain Neoplasms - pathology / Brain tumors / CD45 antigen / Cell surface / Cloning / Emission analysis / Endothelial cells / Female / Gallium / Glioblastoma / Glioblastoma - diagnostic imaging / Glioblastoma - drug therapy / Glioblastoma - pathology / Glioblastoma multiforme / Glioma / Glioma cells / Gliomas / Growth factors / Humans / Immunohistochemistry / Infiltration / Leukocyte Common Antigens - metabolism / Macrophages / Macrophages - immunology / Macrophages - metabolism / Magnetic Resonance Imaging / Male / Medical imaging / Medical prognosis / Metastases / Microglia / Microglial cells / Middle Aged / Nervous system / Neuroimaging / Neuropathology / Neurosurgery / Nuclear medicine / Organometallic Compounds - chemistry / Patients / Peptides / Positron emission / Positron emission tomography / Prognosis / Radiation therapy / Radiography / Receptors, Somatostatin - metabolism / Retrospective Studies / Somatostatin / Somatostatin receptors / Staining / Target recognition / Tomography / Tracers (Biology) / Tumor cells / Tumors