Journal Article

Unravelling the Diversity of Grapevine Microbiome

Sousa, Susana, Pinto, Cátia, Egas, Conceição, Pinho, Diogo, Pinheiro, Miguel, C. Gomes, Ana

2014

Overview

Vitis vinifera is one of the most widely cultivated fruit crops with a great economic impact on the global industry. As a plant, it is naturally colonised by a wide variety of both prokaryotic and eukaryotic microorganisms that interact with grapevine, having either beneficial or phytopathogenic effects, who play a major role in fruit yield, grape quality and, ultimately, in the evolution of grape fermentation and wine production. Therefore, the objective of this study was to extensively characterize the natural microbiome of grapevine. Considering that the majority of microorganisms are uncultivable, we have deeply studied the microflora of grapevine leaves using massive parallel rDNA sequencing, along its vegetative cycle. Among eukaryotic population the most abundant microorganisms belonged to the early diverging fungi lineages and Ascomycota phylum, whereas the Basidiomycota were the least abundant. Regarding prokaryotes, a high diversity of Proteobacteria, Firmicutes and Actinobacteria was unveiled. Indeed, the microbial communities present in the vineyard during its vegetative cycle were shown to be highly structured and dynamic. In all cases, the major abundant microorganisms were the yeast-like fungus Aureobasidium and the prokaryotic Enterobacteriaceae. Herein, we report the first complete microbiome landscape of the vineyard, through a metagenomic approach, and highlight the analysis of the microbial interactions within the vineyard and its importance for the equilibrium of the microecosystem of grapevines.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Agriculture / Analysis / Ascomycota - genetics / Basidiomycota - genetics / Biodiversity / Biology / Biotechnology / Botrytis cinerea / Crop yield / Cultivation / Deoxyribonucleic acid / DNA / DNA, Bacterial - genetics / DNA, Fungal - genetics / DNA, Ribosomal Spacer - genetics / Economic impact / Ecosystems / Enterobacteriaceae - genetics / Fermentation / Food / Fruit crops / Fruit cultivation / Fruits / Fungi / Genomics / Grapes / Grapevines / Impact analysis / International economic relations / Leaves / Metabolites / Microbial activity / Microbiota (Symbiotic organisms) / Microbiota - genetics / Microflora / Microorganisms / Molecular Typing / Mycological Typing Techniques / Plant diseases / Population / Prokaryotes / Proteins / Pseudomonadaceae - genetics / Seasons / Sphingomonadaceae - genetics / Streptococcaceae - genetics / Vineyards / Vitaceae / Vitis - growth & development / Vitis - microbiology / Vitis vinifera / Wine / Wineries / Wineries & vineyards / Wines / Yeast