Journal Article

Environmental Particulate Matter Induces Murine Intestinal Inflammatory Responses and Alters the Gut Microbiome

Hotte, Naomi, Tso, Robert, Kish, Lisa, Gänzle, Michael, Madsen, Karen L., Kaplan, Gilaad G., Rioux, Kevin P., Wine, Eytan, Barkema, Herman W., Vincent, Renaud, Thiesen, Aducio

2013

Overview

Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal and inflammatory conditions. Wild-type 129/SvEv mice were gavaged with Ottawa urban PM10 (EHC-93) for 7-14 days and mucosal gene expression analyzed using Ingenuity Pathways software. Intestinal permeability was measured by lactulose/mannitol excretion in urine. At sacrifice, segments of small and large intestine were cultured and cytokine secretion measured. Splenocytes were isolated and incubated with PM10 for measurement of proliferation. Long-term effects of exposure (35 days) on intestinal cytokine expression were measured in wild-type and IL-10 deficient (IL-10(-/-)) mice. Microbial composition of stool samples was assessed using terminal restriction fragment length polymorphism. Short chain fatty acids were measured in caecum. Short-term treatment of wild-type mice with PM10 altered immune gene expression, enhanced pro-inflammatory cytokine secretion in the small intestine, increased gut permeability, and induced hyporesponsiveness in splenocytes. Long-term treatment of wild-type and IL-10(-/-) mice increased pro-inflammatory cytokine expression in the colon and altered short chain fatty acid concentrations and microbial composition. IL-10(-/-) mice had increased disease as evidenced by enhanced histological damage. Ingestion of airborne particulate matter alters the gut microbiome and induces acute and chronic inflammatory responses in the intestine.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Air Pollutants - adverse effects / Air pollution / Airborne particulates / Analysis / Animals / Biology / Chains / Colon / Colon - immunology / Colon - metabolism / Colon - microbiology / Cytokines / Cytokines - metabolism / Digestive system / Digestive tract / Environmental Exposure - adverse effects / Excretion / Fatty acids / Fatty Acids, Volatile - metabolism / Female / Gastrointestinal tract / Gene expression / Gene Expression Regulation / Gene Regulatory Networks / Genetic aspects / Health aspects / Immune response / Inflammation / Inflammation - etiology / Inflammatory bowel disease / Ingestion / Interleukin 10 / Intestinal microflora / Intestinal Mucosa - metabolism / Intestine / Intestine, Small - immunology / Intestine, Small - metabolism / Intestine, Small - microbiology / Intestines - immunology / Intestines - microbiology / Intestines - pathology / Lactulose / Large intestine / Long-term effects / Mannitol / Medicine / Membrane permeability / Mice / Mice, Knockout / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Mucosa / Oral administration / Oxidative stress / Particulate emissions / Particulate matter / Particulate Matter - administration & dosage / Particulate Matter - adverse effects / Particulate pollutants / Particulates / Permeability / Physiological aspects / Pollutants / Polymorphism / Restriction fragment length polymorphism / Rodents / Small intestine / Spleen - cytology / Spleen - immunology / Splenocytes / Studies / Time Factors / Urban environments / Urine